Characterization of Replication fork DNA specific motor proteins using single molecule analysis

• Project/Area Number: 23770204
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: Kyoto University
• Principal Investigator: HAN Yong-woon 京都大学, 物質-細胞統合システム拠点, 助教 (50566297)
• Research Collaborator: IWASA Takuma
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: DNA複製 / 一分子解析 / ナノ開口基板 / DNAヘリケース / ヌクレオソーム / DNA蛍光標識

Research Abstract

Single molecule imaging techniques enable us to measure and regulate movements of proteins or DNA. Using these techniques, I characterized E. coli UvrD helicase and RuvB motor protein, which promote branch migration of Holliday junction DNA. I also measured association and dissociation rates of Pyrrole-Imidazole polyamides and calculated their model structures to clarify the relationship between the DNA binding kinetics and Pyrrole-Imidazole polyamide structures.

Research Products

• [Journal Article] ParA-mediated plasmid partition driven by protein pattern self-organization (2013)
  • Author(s): Ling-Chin Hwang、Anthony G. Vecchiarelli、Yong-Woon Han、Michiyo Mizuuchi、Yoshie Harada、Barbara E. Funnell and Kiyoshi Mizuuchi
  • Journal Title: The EMBO Journal Volume: 32巻 Issue: 9 Pages: 1238-1249
  • DOI: 10.1038/emboj.2013.34
  • Peer Reviewed
• [Journal Article] Binding of hairpin pyrrole and imidazole polyamides to DNA : relationship between torsion angle and association rate constants (2012)
  • Author(s): Han YW, Matsumoto T, Yokota H, Kashiwazaki G, Morinaga H, Hashiya K, Bando T, Harada Y, Sugiyama H.
  • Journal Title: Nucleic Acids Res Volume: 40 Issue: 22 Pages: 11510-11517
  • DOI: 10.1093/nar/gks897
  • Peer Reviewed
• [Journal Article] 環状ピロール-イミダゾールポリアミドの合成とDNA認識能 (2012)
  • Author(s): 韓 龍雲
  • Journal Title: ファルマシア Volume: 48巻 Pages: 795-795
  • NAID: 110009901169
• [Journal Article] 環状ピロール―イミダゾールポリアミドの合成とDNA認識能 (2012)
  • Author(s): 韓 龍雲 （韓 龍雲）
  • Journal Title: ファルマシア Volume: 48 Pages: 795-795
  • NAID: 110009901169
• [Presentation] Characterization of SRA-mediated DNA complexes dynamics related to chromatin structure regulation (2013)
  • Author(s): Yong-Woon Han、Hiroaki Yokota、Mariko Ariyoshi、Yasuo Tsunaka、Takuma Iwasa、Ryuji Yokokawa、Ryo Hiramatsu、Daichi Chiba、Teruo Ono、Yoshie Harada
  • Organizer: The 2013 Biophysical Society 57th Annual Meeting
  • Place of Presentation: フィラデルフィア、米国
• [Presentation] ナノ開口基板を用いたSRAドメインのヘミメチルCpG認識機構の解析 (2013)
  • Author(s): 韓 龍雲、横田浩章、有吉真理子、津中康央、岩佐拓磨、平松亮、千葉大地、小野輝男、横川隆司、原田慶恵
  • Organizer: 2013年生体運動研究合同班会議
  • Place of Presentation: 広島大学
• [Presentation] ナノ開口基板を用いたSRAドメインのヘミメチルCpG認識機構の解析 (2013)
  • Author(s): 韓 龍雲 (韓 龍雲)
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 広島大学
• [Presentation] Characterization of SRA-methylated DNA complexes dynamics related to chromatin structure regulation (2013)
  • Author(s): Yong-Woon Han (Yong-Woon Han)
  • Organizer: The 2013 Biophysical Society 57th Annual Meeting
  • Place of Presentation: フィラデルフィア、米国
• [Presentation] GCGC配列を認識するヘアピン型ピロール-イミダゾールポリアミドのDNAに対する親和性はピロールをβ-アラニンに置換することで向上する (2012)
  • Author(s): 韓 龍雲、横田浩章、松本朋子、森永浩伸、柏崎玄伍、橋谷かおり、坂東俊和、原田慶恵、杉山 弘
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Funnell、Plasmid partition and cell division control via ATP-driven protein distribution pattern self-organization on bacterial intracellular surfaces (2012)
  • Author(s): Mizuuchi Kiyoshi、Anthony G. Vicchiarelli、Ling-Chin Hwang、Keir Neumann、Yong-Woon Han、Arianna Biesso、Vassili Ivanov、Yoshie Harada、Barbara E
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Characterization of protein-DNA complexes dynamics related to chromatin structure regulation using single-molecule techniques (2012)
  • Author(s): Yong-Woon Han、Yoshie Harada
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋大学
• [Presentation] DNA塩基配列特異的に結合する小化合物Pyrrole-Imidazole Polyamide におけるPyrrole と Imidazole の構造学的特徴 (2012)
  • Author(s): 韓 龍雲、松本朋子、横田浩章、柏崎玄伍、森永浩伸、橋谷かおり、坂東俊和、杉山弘、原田慶恵
  • Organizer: 2012年生体運動研究合同班会議
  • Place of Presentation: 筑波大学
• [Presentation] Characterization of protein-DNA complexes dynamics related to chromatin structure regulation using single-molecule techniques (2012)
  • Author(s): Yong-Woon Han (Yong-Woon Han)
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 名古屋大学
  • Invited
• [Presentation] Plasmid partition and cell division control via ATP-driven protein distribution pattern self-organization on bacterial intracellular surfaces (2012)
  • Author(s): Mizuuchi Kiyoshi (Yong-Woon Han)
  • Organizer: 分子生物学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] GCGC配列を認識するヘアピン型ピロール-イミダゾールポリアミドのDNAに対する親和性はピロールをβ-アラニンに置換することで向上する (2012)
  • Author(s): 韓 龍雲 (韓 龍雲)
  • Organizer: 分子生物学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Vecchiarelli、Michiyo Mizuuchi, Barbara E. Funnell、Yoshie Harada、Kiyoshi Mizuuchi、Direct observation of P1 plasmid movement on an optical microscope (2011)
  • Author(s): Yong-Woon Han、Ling-Chin Hwang, Anthony G
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学
• [Presentation] Single-molecule visualization of a AAA+ DNA recombination ATPase with zero-mode waveguides toward elucidation of its hexamer formation (2011)
  • Author(s): Takuma Iwasa、Yong-Woon Han、Hiroaki Yokota、Ryuji Yokokawa、Yoshie Harada
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学
• [Remarks]
  • URL: http://www.harada.icems.kyoto-u.ac.jp/member/mem03_han.html