Characterization of Replication fork DNA specific motor proteins using single molecule analysis
| Project/Area Number |
23770204
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Kyoto University |
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Principal Investigator |
HAN Yong-woon 京都大学, 物質-細胞統合システム拠点, 助教 (50566297)
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| Research Collaborator |
IWASA Takuma
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | DNA複製 / 一分子解析 / ナノ開口基板 / DNAヘリケース / ヌクレオソーム / DNA蛍光標識 |
|---|
| Research Abstract |
Single molecule imaging techniques enable us to measure and regulate movements of proteins or DNA. Using these techniques, I characterized E. coli UvrD helicase and RuvB motor protein, which promote branch migration of Holliday junction DNA. I also measured association and dissociation rates of Pyrrole-Imidazole polyamides and calculated their model structures to clarify the relationship between the DNA binding kinetics and Pyrrole-Imidazole polyamide structures.
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Report
(3 results)
Research Products
(18 results)
