The regulation mechanism of protein biosynthesis on ribosome in the hibernation stage
| Project/Area Number |
23770206
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Osaka University |
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Principal Investigator |
KATO Takayuki 大阪大学, 生命機能研究科, 助教 (20423155)
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| Research Collaborator |
MIYATA Tomoko 大阪大学, 生命機能研究科, 特任助教 (30423156)
YOSHIDA Hideji 大阪医科大学, 基礎医学, 准教授 (60288735)
UETA Masami 吉田生物研究所, 研究員 (30512511)
WADA Akira 吉田生物研究所, 室長 (80025387)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 100S リボソーム / 飢餓的ストレス / 低温電子顕微鏡 / 単粒子解析 / 100Sリボソーム / 単粒子像解析 / 細胞休止期 / 構造解析 |
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| Research Abstract |
To understand the regulation mechanism of protein biosysnthesis under the starvation stress, structural analysis of 100S ribosomes was carried out by cryo-EM. In the case of E. coli, 30S subunit inserted to the entrance for mRNA. On the other hand, in the case of Staphylococcus Aureus, the exit site for mRNA was covered each 30S. And we confirmed the binding mode of Erviniawhich has E. coli type HPF and Lactobacilluswhich has Staphylococcustype HPF by cryoEM. So we demonstrated that two types of HPF produces two different types of binding mode of 100S ribosome.
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Report
(3 results)
Research Products
(40 results)
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[Presentation] Structural analysis of 100S ribosomes in two different types of bacteria.2013
Author(s)
Kato, T., Ueta, M., Miyata, T., Wada, C., Yoshida, H., Wada, A. & Namba, K.
Organizer
Nagoya Symposium -Frontiers in Structural Physiology-.
Place of Presentation
Nagoya University(愛知県・名古屋市)
Related Report
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[Presentation] 飢餓的ストレス条件下における細菌の生存戦略2012
Author(s)
加藤貴之, 上田雅美, 宮田知子, 吉田秀司, 和田千恵子, 和田明, 難波啓一
Organizer
第35回日本分子生物学会年会
Place of Presentation
福岡国際会議場・マリンメッセ福岡(福岡県・福岡市)
Year and Date
2012-12-13
Related Report
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[Presentation] 飢餓的ストレス下での100S リボソーム形成による細菌の生存戦略.Survival strategy ofbacteria under the starvation stress by 100S ribosome formation.2012
Author(s)
Kato, T., Ueta, M., Miyata, T., Yoshica, H., Wada, A., Namba, K.
Organizer
第50回日本生物物理学会年会.
Place of Presentation
名古屋大学(愛知県・名古屋市)
Year and Date
2012-09-24
Related Report
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[Presentation] 飢餓的ストレス下における細菌の生存戦略(The survival strategy in bacteria under the starvation stress).2011
Author(s)
Kato, T., Ueta, M., Miyata, T., Yoshida, H., Maki, Y., Furuike, S., Sakai, A., Wada, A., Namba, K.
Organizer
日本生物物理学会第49回年会
Place of Presentation
兵庫県立大学・姫路書写キャンパス(兵庫県・姫路市)
Year and Date
2011-09-18
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