Analysis of maturation process of endo -siRNA precursor

• Project/Area Number: 23770210
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: Niigata University
• Principal Investigator: MIYOSHI Tomohiro 新潟大学, 研究推進機構超域学術院, 助教 (60534550)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: RNA / siRNA / 小分子RNA / RNAサイレンシング / non-coding RNA / miRNA / プロセシング / Dicer

Research Abstract

Non-coding small RNAs act as functional molecules in cells. Endogenous siRNA (endo-siRNA) isone of the small RNAs, but its biosynthesis process is unknown. In this study, we succeeded indetection of endo-siRNA precursor present in the cell. Further, we showed that novel cytoplasmic fociare functionally important in the endo-siRNA biosynthesis. Furthermore, we identified novel proteinrelated to the biosynthesis mechanism of endo-siRNA.

Research Products

• [Journal Article] Roles of R2D2, a cytoplasmic D2 body component, in the endogenous siRNA pathway in Drosophila. (2013)
  • Author(s): Nishida, KM.
  • Journal Title: Molecular Cell Volume: 49 Issue: 4 Pages: 680-691
  • DOI: 10.1016/j.molcel.2012.12.024
  • Peer Reviewed
• [Journal Article] Analysis of chimeric ribosomal stalk complexes from eukaryotic and bacterial sources: structural features responsible for specificity of translation factors (2012)
  • Author(s): Mochizuki, M. 他
  • Journal Title: Genes to Cells Volume: 17 Issue: 4 Pages: 273-284
  • DOI: 10.1111/j.1365-2443.2012.01586.x
  • Peer Reviewed
• [Presentation] The C-terminal region of the ribosomal stalk protein is required for depurination at the SRL region of rRNA (2012)
  • Author(s): Yusa K , Miyoshi T , Lee KM ,Maruyama R,Ohno M,Ito K,Wong KB,Uchiumi T.
  • Organizer: 2012 Niigata Graduate Research Forum Research Camp
  • Place of Presentation: 朱鷺メッセ
• [Presentation] Evidence for Interaction of the C-terminal Region Shared by Human Ribosomal Stalk Proteins P0/P1/P2 with eEF-1A/eEF-2 (2012)
  • Author(s): Onozuka M, Baba K, Miyoshi T, Ito K,Uchiumi T.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡
• [Presentation] The Hinge Regions of Eukaryotic Ribosomal Stalk Proteins are Indispensable for Efficient Depurination of 28S rRNA by Ribotoxins (2012)
  • Author(s): Yusa K, Ohno M, Miyoshi T, Ito K, Uchiumi T.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡
• [Presentation] Construction of in vitro Translation Elongation System with Archaeal Elongation Factors and Hybrid Ribosomes:Control of Interaction aEF-1A and Stalk aP1 by aEF-1B (2012)
  • Author(s): Suzuki T, Honda T, Sato S, Miyoshi T, Ito K, Uchiumi T.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡
• [Presentation] リボトキシンによるリボソーム不活性化作用への動物リボソームstalkタンパク質の関与 (2012)
  • Author(s): 遊佐 和之，三好 智博，Ka-Ming Lee，大野 萌，伊東 孝祐，Kam-Bo Wong，内海 利男
  • Organizer: 新潟生化学懇話会
  • Place of Presentation: 新潟大学（新潟市）
• [Presentation] Evidence for Interaction of the C-terminal Region Shared by Human Ribosomal Stalk Proteins P0/P1/P2 with eEF-1A/eEF-2 (2012)
  • Author(s): Onozuka, M., Baba, K., Miyoshi, T., Ito, K., Uchiumi, T.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡（福岡市）
• [Presentation] The Hinge Regions of Eukaryotic Ribosomal Stalk Proteins are Indispensable for Efficient Depurination of 28S rRNA by Ribotoxins (2012)
  • Author(s): Yusa, K., Ohno, M., Miyoshi, T., Ito, K., Uchiumi, T.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡（福岡市）
• [Presentation] Construction of in vitro Translation Elongation System with Archaeal Elongation Factors and Hybrid Ribosomes: Control of Interaction aEF-1A and Stalk aP1 by aEF-1B (2012)
  • Author(s): Suzuki, T., Honda, T., Sato, S., Miyoshi, T., Ito, k., Uchiumi,. T.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡（福岡市）
• [Presentation] ショウジョウバエsmallRNA生合成機構の解明 (2011)
  • Author(s): 三好智博
  • Organizer: 新潟生化学懇話会
  • Place of Presentation: 新潟薬科大学
  • Year and Date: 2011-06-25
• [Presentation] リボトキシンによるリボソーム不活性化作用への動物リボソームstalkタンパク質の関与 (2011)
  • Author(s): 遊佐和之,三好智博,Ka-MingLee,大野萌,伊東孝祐,Kam-BoWong,内海利男
  • Organizer: 新潟生化学懇話会
  • Place of Presentation: 新潟大学
• [Presentation] リボトキシンによるrRNA機能部位の脱プリン作用にはリボソームstalkタンパク質のC末端部位が関与する (2011)
  • Author(s): 遊佐和之、三好智博、Ka-Ming Lee、円山亮、大野萌、伊東孝祐、Kam-Bo Wong、内海利男
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Introduction of a Lepidoptera-specific sequence within the GTPase-associated center of 28S rRNA into Escherichia coli 23S rRNA: Effects of the mutations (2011)
  • Author(s): Ryo Maruyama, Kazuyuki Yusa, Masahiro Mochizuki, Tomohiro Miyoshi, Kosuke Ito, Takaomi Nomura, Uchiumi Toshio
  • Organizer: 日本RNA学会
  • Place of Presentation: 京都国際会議場
• [Remarks]
  • URL: http://www.sc.niigata-u.ac.jp/biologyindex/uchiumi-ito/index.html