Quality control of Rad51-nucleoprotein filaments in HR

• Project/Area Number: 23770212
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: Tokyo Institute of Technology (2012); National Institute of Genetics (2011)
• Principal Investigator: KUROKAWA Yumiko 東京工業大学, 情報生命博士教育院, 特任助教 (10381633)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 組換え / 相同組換え / 修復 / DNA / Rad51 / へリカーゼ / ユビキチン / ヘリカーゼ

Research Abstract

In wild type cells, homologous recombination (HR) is required for repair of double-stranded DNA breaks (DSBs). If not processed properly, DSBs could cause detrimental effects in cells such as chromosome rearrangement, cell death, tumorigenesis and so on. HR, therefore, must be tightly regulated. To clarify the molecular mechanism of regulating HR, we have characterized Rad51-mediated reactions in vitro with severalfactors (activators, mediators, helicases and ubiquitin systems).

Research Products

• [Journal Article] Yanagida Opposing Role of Condensin Hinge against RPA in Mitosis and Interphase through Promoting DNA Annealing. (2011)
  • Author(s): Y. Akai, Y. Kurokawa, N. Nakazawa, Y. Tonami- Murakami, Y. Suzuki, S. H. Yoshimura, H. Iwasaki, Y. Shiroiwa, T. Nakamura, E. Shibata and M.
  • Journal Title: Open Biology Volume: 1巻
  • Peer Reviewed
• [Journal Article] Opposing role of condensing hinge against replication protein A in mitosis and interphase through promoting DNA annealing (2011)
  • Author(s): Yuko Akai, Yumiko Kurokawa, Norihiko Nakazawa, Yuko Tonami-Murakami, Yuki Suzuki, Shige H.Yoshimura, Hiroshi Iwasaki, Yoshiharu Shiroiwa, Takahiro Nakamura, Eri Shibata, Mitsuhiro Yanagida
  • Journal Title: Open Biology Volume: 1 Issue: 4 Pages: 110023-110023
  • DOI: 10.1098/rsob.110023
  • Peer Reviewed
• [Presentation] 分裂酵母Rad51リコンビナーゼのHis-315ミュータントの解析 (2012)
  • Author(s): 伊藤健太郎、筒井康博、真柳浩太、黒川裕美子、小甲裕一、池口満徳、山尾文明、岩崎博史
  • Organizer: 日本遺伝学会
  • Place of Presentation: 九州大学医学部(福岡県)
  • Year and Date: 2012-09-26
• [Presentation] 分裂酵母Rad51リコンビナーゼのHis-315ミュータントの解析 (2011)
  • Author(s): 伊藤健太郎、黒川裕美子、筒井康博、小甲裕一、池口満徳、山尾文明、岩崎博史
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-15
• [Presentation] 分裂酵母 Rad51 リコンビナーゼの His-315 ミュータントの解析 (2011)
  • Author(s): 伊藤健太郎、黒川裕美子、筒井康博、小甲裕一、池口満徳、山尾文明、岩崎博史
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] 分裂酵母Rad51リコンビナーゼのHis-315ミュータントの解析
  • Author(s): 伊藤健太郎、筒井康博、真柳浩太、黒川裕美子、小甲裕一、池口満徳、山尾文明、岩崎博史
  • Organizer: 日本遺伝学会
  • Place of Presentation: 九州大学医学部