| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
Hamlet is the evolutionarily conserved Drosophila Prdm transcription factor family member and it has been demonstrated that Hamlet controls non-neural versus neural cell fate in the Drosophila melanogaster External Sensory Organ lineage (Moore et al. Science. 2002 297:1355-8). By means of chromatin immunoprecipitation and proteomic approaches, I elucidated that Hamlet modifies histone density and histone methylation at Notch target gene loci. Hamlet contains two zinc finger clusters; a PR (PRDI-BF1 and RIZ homology) domain, a derivative of the SET domain; and a C-terminal Binding Protein (CtBP) transcriptional co-repressor binding site. All these domains are highly conserved in HAM homologues from C. elegans (Egl43) to mouse and humans (Prdm16). By using transgenic flies which lack these conserved Hamlet domains, we determined that both zinc finger clusters and CtBP domains are required for proper Hamlet function.
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