Search for novel membrane trafficking pathway involved in autophagy
| Project/Area Number |
23770216
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Tohoku University |
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Principal Investigator |
ITOH Takashi 東北大学, 大学院・生命科学研究科, 助教 (50373270)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | タンパク質分解 / 膜輸送 / オートファジー / Rab / Rab-GAP |
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| Research Abstract |
Macroautpohagy (referred to as autophagy hereafter) accompanies dynamic membrane remodeling such as autophagosome formation and fusion between autophagosome and lysosome. However, the regulation of such membrane remodeling is largely unknown. Since I had identified three Rab-GAPs, inactivators of Rab-type small GTPases, as autophagosome resident proteins, here I addressed the function of these Rab-GAPs (OATL1/TBC1D25, TBC1D2B, TBC1D11) in autophagy. They were localized at autophagosomes and bound with LC3, an autophagosome resident protein used as a marker of autophagosome. TBC1D2B and TBC1D25 required the interaction with LC3 for their localization at autophagosomes, since a point mutation that abolished the interaction impaired their localization. Although I previously revealed that overexpression of TBC1D25 inhibits fusion between autophagosomes and lysosomes, overexpression of TBC1D2B nor TBC1D11 did not inhibit any process of autophagy.
These results so far did not clarify relationship between these Rab-GAPs and autophagy. However, I suppose that it is possible that LC3 acts as a scaffold for Rab-GAPs and has a role in membrane trafficking in addition to in autophagy.
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Report
(3 results)
Research Products
(9 results)
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[Journal Article] Atg16L2, a novel isoform of mammalian Atg16L that is not essential for canonical autophagy despite forming an At12-5-16L2 complex2011
Author(s)
Ishibashi, K., Fujita, N., Kanno, E., Omori, H.,Yoshimori, T., Itoh, T .& Fukuda. M
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Journal Title
Autophagy
Volume: 7
Issue: 12
Pages: 1500-1513
DOI
Related Report
Peer Reviewed
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[Presentation] A possible regulation of Rab33B-dependent membrane trafficking by the Atg12-5/16L1 complex2012
Author(s)
Itoh, T., Fujita, N., Saitoh, T., Komatsu, M, Akira, S., Yoshimori, T., and Fukuda, M.
Organizer
第34回日本分子生物学会年会
Place of Presentation
福岡
Year and Date
2012-12-13
Related Report
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[Presentation] A possible regulation of Rab33B-dependent membrane trafficking by the Atg12-5/16L1 complex.2012
Author(s)
Itoh, T., Fujita, N., Saitoh, T., Komatsu, M, Akira, S., Yoshimori, T., and Fukuda, M.
Organizer
第34回 日本分子生物学会年会
Place of Presentation
福岡
Related Report
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