Investigation of the novel activation mechanism of MRTF, a master transcriptional regulator of the actin cytoskeleton.
| Project/Area Number |
23770225
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Osaka University |
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Principal Investigator |
MORITA Tsuyoshi 大阪大学, 大学院・医学系研究科, 助教 (80403195)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 細胞骨格 / 運動 / 転写因子 / ガン / 平滑筋 / 平滑筋分化 / 癌 |
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| Research Abstract |
In this study, I investigated the novel activation mechanism of myocardin family proteins, myocardin and MRTF. The activity of MRTF is suppressed by interaction with actin. I identified Tb4 protein as an inhibitory factor of MRTF/actin interaction. myocardin also interacts with actin, but its affinity for actin is rather low. I demonstrated that actin-related protein 5 (Arp5), instead of actin, tightly bound to myocardin and suppressed its activity. In smooth muscle cells, Arp5 expression level is very low, resulting in high activity of myocardin.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] RPEL proteins are the molecular targets for CCG-1423, an inhibitor of Rho signaling.2014
Author(s)
Hayashi, K., Watanabe, B., Nakagawa, Y., Minami, S., and Morita, T.
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Journal Title
PLOS ONE
Volume: 9
Issue: 2
Pages: e89016-e89016
DOI
Related Report
Peer Reviewed
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