Analysis of autophagosome formation using CLEM tomography

• Project/Area Number: 23770226
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: HAMASAKI Maho 大阪大学, 医学系研究科, 助教 (30455216)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: 生体膜 / オルガネラ / オートファジー / 小胞体 / ミトコンドリア / membrane trafficking / オートファゴソーム / 電子顕微鏡 / トモグラフィー

Research Abstract

One of the cell survival mechanism called autophagy gets activated when cells face starved condition, accumulates abnormal proteins, bacterial invasion and so on. Autophagosome (AP) is formed newly within cytosol that gets fused with lysosomes for degradation. Where AP is formed within cells have been a long-standing question since that will leads to its membrane source. We identified such site to be ER-mitochondria contact site (Nature, 2013). AP formation is dynamic and this finding will help characterizing the most interesting part of formation.

Research Products

• [Journal Article] Up-to-date membrane biogenesis in the autophagosome formation (2013)
  • Author(s): Hamasaki M, Shibutani ST, Yoshimori T
  • Journal Title: Current Opinion in Cell Biology Volume: 25 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Autophagosomes form at ER-mitochondria contact sites (2013)
  • Author(s): Hamasaki M, Furuta N, Matsuda A, Nezu A, Yamamoto A, Fujita N, Oomori H, Noda T, Haraguchi T, Hiraoka Y, Amano A, Yoshimori T
  • Journal Title: Nature Volume: 495(7441) Pages: 389-393
  • Peer Reviewed
• [Journal Article] Up-to-date membrane biogenesis in the autophagosome formation (2013)
  • Author(s): Hamasaki M, Shibutani ST, Yoshimori T.
  • Journal Title: Current Opinion in Cell Biology Volume: N/A
  • Peer Reviewed
• [Presentation] Autophagosomes form at ER-mitochondria contact sites (2013)
  • Author(s): Hamasaki M, Furuta N, Matsuda A, Nezu A, Yamamoto A, Fujita N, Oomori H, Noda T, Haraguchi T, Hiraoka Y, Amano A, Yoshimori T
  • Organizer: EMBO autophagy
  • Place of Presentation: Norway
• [Presentation] Autophagosomes form at ER-mitochondria contact sites (2013)
  • Author(s): Hamasaki M, Furuta N, Matsuda A, Nezu A, Yamamoto A, Fujita N, Oomori H, Noda T, Haraguchi T, Hiraoka Y, Amano A, Yoshimori T
  • Organizer: Quantitative Bioimaging
  • Place of Presentation: Okazaki
• [Presentation] The autophagosome formation takes place at ER-mitochondria contact sites (2013)
  • Author(s): 1,2M. Hamasaki, 3,4N.Furuta, 1,2N.Fujita, 5H.Omori, 1,2A.Nezu, 1,2T.Noda, 6A.Yamamoto, 3,4A.Amano, 1,2T.Yoshimori
  • Organizer: Quantitative Bioimaging
  • Place of Presentation: Okazaki
• [Presentation] Autophagosomes form at ER-mitochondria contact sites (2012)
  • Author(s): Hamasaki M, Furuta N, Matsuda A, Nezu A, Yamamoto A, Fujita N, Oomori H, Noda T, Haraguchi T, Hiraoka Y, Amano A, Yoshimori T
  • Organizer: ASCB (American society for Cell Biology)
  • Place of Presentation: San Francisco
• [Presentation] Autophagosomes form at ER-mitochondria contact sites (2012)
  • Author(s): Hamasaki M, Furuta N, Matsuda A, Nezu A, Yamamoto A, Fujita N, Oomori H, Noda T, Haraguchi T, Hiraoka Y, Amano A, Yoshimori T
  • Organizer: ISA (International Symposium on Autophagy)
  • Place of Presentation: Okinawa
• [Presentation] Autophagosomes form at ER-mitochondria contact sites (2012)
  • Author(s): Hamasaki M, Furuta N, Matsuda A, Nezu A, Yamamoto A, Fujita N, Oomori H, Noda T, Haraguchi T, Hiraoka Y, Amano A, Yoshimori T
  • Organizer: The 4^<th> EMBO meeting
  • Place of Presentation: Nice
• [Presentation] The autophagosome formation takes place at ER-mitochondria junction (2012)
  • Author(s): 1,2M. Hamasaki, 3,4N.Furuta, 1,2N.Fujita, 5H.Omori, 1,2A.Nezu, 1,2T.Noda, 6A.Yamamoto, 3,4A.Amano, 1,2T.Yoshimori
  • Organizer: the 4th EMBO meeting
  • Place of Presentation: Nice
• [Presentation] The autophagosome formation takes place at ER-mitochondria junction (2012)
  • Author(s): 1,2M. Hamasaki, 2,3A. Matsuda, 1,2A.Nezu, 3T. Haraguchi, 2Y. Hiraoka and 1,2T.Yoshimori
  • Organizer: ISA (International Symposium on Autophagy)
  • Place of Presentation: Okinawa
  • Invited
• [Presentation] The autophagosome formation takes place at ER-mitochondria contact sites (2012)
  • Author(s): 1,2M. Hamasaki, 3,4N.Furuta, 1,2N.Fujita, 5H.Omori, 1,2A.Nezu, 1,2T.Noda, 6A.Yamamoto, 3,4A.Amano, 1,2T.Yoshimori
  • Organizer: ASCB (American society for Cell Biology
  • Place of Presentation: San Francisco
• [Presentation] オートファゴソーム膜形成過程における液胞膜の関与の解明 (2011)
  • Author(s): 濱崎万穂、Marko Kaksonen, 大隅良典、吉森保
  • Organizer: EMBO meeting
  • Place of Presentation: Austria Center Vienna(ACV), ウィーン, オーストリア
• [Book] オートファゴソーム膜の起源の謎 (2013)
  • Author(s): 濱崎万穂、吉森保
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/yoshimori/
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/yoshimori/jp/achievement/010/