Role of FBP17,an EFC/F-BAR protein, in phagocytic cupformation

• Project/Area Number: 23770228
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Kobe University
• Principal Investigator: TSUJITA Kazuya 神戸大学, 大学院・医学研究科, 助教 (10457054)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: F-BAR / ドメイン / アクチン重合 / F-BAR ドメイン / 細胞膜 / F-BAR domain / アクチン細胞骨格 / phagocytosis

Research Abstract

By performing in vitro analysis, we directly showed the mechanism by which FBP17 regulates actin polymerization on the membrane tubules. Physiologically, it was found that FBP17 is recruited to phagocytic cup via its F-BAR domain and that this accumulation is critical for phagocytic cup formation. Furthermore, RNAi and its rescue experiments revealed that the assembly of FBP17 at the plasma membrane is dependent on its F-BAR domain, promoting actin polymerization at phagocytic cup membrane. These data suggest that invaginated membrane caused by FBP17 may function as a scaffold for actin polymerization.

Research Products

• [Journal Article] Physicochemical analysis from real-time imaging of liposome tubulation reveals the characteristics of individual F-BAR domain proteins. (2013)
  • Author(s): Tanaka-Takiguchi Y, Itoh T, Tsujita K, Yamada S, Yanagisawa M, Fujiwara K, Yamamoto A, Ichikawa M, Takiguchi K
  • Journal Title: Langmuir. Volume: 29 Pages: 328-336
• [Journal Article] Antagonistic regulation of F-BAR protein assemblies controls actin polymerization during podosome formation. (2013)
  • Author(s): Tsujita K, Kondo A, Kurisu S, Hasegawa J, Itoh T, Takenawa T.
  • Journal Title: Journal of Cell Science Volume: In press Pages: 2267-2278
  • DOI: 10.1242/jcs.122515
  • Peer Reviewed
• [Journal Article] Physicochemical analysis from real-time imaging of liposome tabulation reveals the characteristic of individual F-BAR domain proteins (2013)
  • Author(s): Takiguchi Y, Itoh T, Tsujita K, Yamada S, Yanagisawa M, Fujiwara K, Ichikawa M, Yamamoto A, Takiguchi K
  • Journal Title: Langmuir Volume: 29 Issue: 1 Pages: 328-336
  • DOI: 10.1021/la303902q
  • Peer Reviewed
• [Journal Article] ARAP1 regulates the ring size of circular dorsal ruffles through Arf1 and Arf5 (2012)
  • Author(s): Hasegawa J, Tsujita K, Takenawa T, Itoh T.
  • Journal Title: Molecular Biology of The Cell Volume: 23 Pages: 2481-2489
• [Journal Article] ARAP1 regulates the ring size of circular dorsal ruffles through Arf1 and Arf5 (2012)
  • Author(s): Hasegawa J
  • Journal Title: Mol. Biol. Cell Volume: 23 Issue: 13 Pages: 2481-2489
  • DOI: 10.1091/mbc.e12-01-0017
  • Peer Reviewed
• [Journal Article] Characterization of the EFC/F-BAR domain protein (2011)
  • Author(s): Uezu A, Umeda K, Tsujita K, Suetsugu S, Takenawa T, Nakanishi H
  • Journal Title: CHO2.Genes to Cell Volume: 8 Pages: 868-878
• [Journal Article] SH3YL1 regulates dorsal ruffle formation by a novel phosphoinositide-binding domain (2011)
  • Author(s): Hasegawa J, Tokuda E, Tenno T, TsujitaK, Sawai H, Hiroaki H, Takenawa T, Itoh T.
  • Journal Title: Journal of Cell Biology Volume: 193 Pages: 901-916
• [Journal Article] Characterization of the EFC/F-BAR domain protein, FCHO2. (2011)
  • Author(s): Uezu A, Umeda K, Tsujita K, Suetsugu S, Takenawa T, Nakanishi H.
  • Journal Title: Genes to cells Volume: 8 Pages: 868-878
  • Peer Reviewed
• [Journal Article] SH3YL1 regulates dorsal ruffle formation by a novel phosphoinositide-binding domain (2011)
  • Author(s): Hasegawa J, Tokuda E, Tenno T, Tsujita K, Sawai H, Hiroaki H, Takenawa T, Itoh T.
  • Journal Title: The Journal of Cell Biology Volume: 193 Pages: 901-916
  • Peer Reviewed
• [Journal Article] Antagonistic regulation of F-BAR protein assemblies controls actin polymerization during podosome formation
  • Author(s): Tsujita K,Kondo A, Kurisu S, Hasegawa J, Itoh T, Takenawa T.
  • Journal Title: Journal of Cell Science
• [Presentation] Antagonistic regulation of F-BAR domain assembly on membrane. (2011)
  • Author(s): 辻田 和也
  • Organizer: 第84回日本生化学会大会(口頭発表)
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-09-23
• [Presentation] Regulation of membrane mediated actin assembly by EFC/F-BAR proteins. (2011)
  • Author(s): 辻田 和也
  • Organizer: 第63回日本細胞生物学会大会(口頭発表)
  • Place of Presentation: 北海道大学
  • Year and Date: 2011-06-29