Role of FBP17,an EFC/F-BAR protein, in phagocytic cupformation
| Project/Area Number |
23770228
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Kobe University |
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Principal Investigator |
TSUJITA Kazuya 神戸大学, 大学院・医学研究科, 助教 (10457054)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | F-BAR / ドメイン / アクチン重合 / F-BAR ドメイン / 細胞膜 / F-BAR domain / アクチン細胞骨格 / phagocytosis |
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| Research Abstract |
By performing in vitro analysis, we directly showed the mechanism by which FBP17 regulates actin polymerization on the membrane tubules. Physiologically, it was found that FBP17 is recruited to phagocytic cup via its F-BAR domain and that this accumulation is critical for phagocytic cup formation. Furthermore, RNAi and its rescue experiments revealed that the assembly of FBP17 at the plasma membrane is dependent on its F-BAR domain, promoting actin polymerization at phagocytic cup membrane. These data suggest that invaginated membrane caused by FBP17 may function as a scaffold for actin polymerization.
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Report
(3 results)
Research Products
(12 results)
