Mechanism of heterochromat i n formation during early mouse development

• Project/Area Number: 23770235
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: National Institute of Genetics
• Principal Investigator: HIRATANI Ichiro 国立遺伝学研究所, 構造遺伝学研究センター, 助教 (40583753)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 核構造 / クロマチン / 生物・生体工学 / ゲノム / 遺伝子 / 発生・分化

Research Abstract

Earlier studies suggested that an extensive heterochromatin formation takes place throughout the genome in post-implantation mouse embryos (heterochromatin: condensed chromosome regions with transcriptionally inactive genes). To explore its significance, we set out to identify genes involved in this heterochromatinization process. A list of candidate genes was generated and we asked whether suppression of any of these candidate genes could disrupt heterochromatin formation. This led to the identification of four novel genes involved in this process.

Research Products

• [Journal Article] Chromosome engineering allows the efficient isolation of vertebrate neocentromeres (2013)
  • Author(s): Fujiyama A, Kimura H. Earnshaw WC, Fukagawa T
  • Journal Title: Developmental Cell Volume: 24 Pages: 635-648
  • Peer Reviewed
• [Journal Article] Chromosome engineering allows the efficient isolation of vertebrate neocentromeres (2013)
  • Author(s): Shang WH, Hori T, Martins NM, Toyoda A, Misu S, Monma N, Hiratani I, Maeshima K, Ikeo K, Fujiyama A, Kimura H, Earnshaw WC, and Fukagawa T
  • Journal Title: Dev Cell Volume: 24 Issue: 6 Pages: 635-648
  • DOI: 10.1016/j.devcel.2013.02.009
  • Peer Reviewed
• [Journal Article] DNA replication timing is maintained genome-wide in primary human myoblasts independent of D4Z4 contraction in FSH muscular dystrophy (2011)
  • Author(s): Pope BD, Tsumagari K, Battaglia D, Ryba T, Hiratani I, Ehrlich M, Gilbert DM
  • Journal Title: PLoS One Volume: 6
  • Peer Reviewed
• [Journal Article] Replication timing: a fingerprint for cell identity and pluripotency (2011)
  • Author(s): Ryba T, Hiratani I, Sasaki T, Battaglia D, Kulik M, Zhang J, Dalton S, Gilbert DM
  • Journal Title: PLoS Computational Biology Volume: 7
  • Peer Reviewed
• [Journal Article] Genome-scale analysis of replication timing: from bench to bioinformatics (2011)
  • Author(s): Ryba T, Battaglia D, Pope BD. Hiratani I, Gilbert DM
  • Journal Title: Nature Protocols Volume: 6 Pages: 870-895
  • Peer Reviewed
• [Journal Article] Replication timing: a fingerprint for cell identity and pluripotency (2011)
  • Author(s): Ryba T. et al.
  • Journal Title: PLoS Computational Biology Volume: 7 Issue: 10 Pages: e1002225-e1002225
  • DOI: 10.1371/journal.pcbi.1002225
  • Peer Reviewed
• [Journal Article] DNA replication timing is maintained genome-wide in primary human myoblasts independent of D4Z4 contraction in FSH muscular dystrophy (2011)
  • Author(s): Pope B. D. et al.
  • Journal Title: PLoS One Volume: 6 Issue: 11 Pages: e27413-e27413
  • DOI: 10.1371/journal.pone.0027413
  • Peer Reviewed
• [Presentation] Developmental regulation of nuclear genome organization (2013)
  • Author(s): 平谷 伊智朗
  • Organizer: RSC Publishing-iCeMS Joint International Symposium "Cell-Material Integration and Siomaterials Science"(国際シンポジウム)
  • Place of Presentation: 京都(京都大学医学部芝蘭会館)
• [Presentation] Developmental regulation of nuclear genome organization (2013)
  • Author(s): 平谷 伊智朗
  • Organizer: RSC Publishing-iCeMS Joint International Symposium "Cell-Material Integration and Biomaterials Science"
  • Place of Presentation: 京都（京都大学医学部芝蘭会館）
  • Invited
• [Presentation] Developmental regulation of nuclear genome organization (2012)
  • Author(s): 平谷 伊智朗
  • Organizer: 第35回 日本分子生物学会年会
  • Place of Presentation: 福岡(福岡国際会議場・マリンメツセ福岡
• [Presentation] Developmental regulation of nuclear genome organization (2012)
  • Author(s): 平谷 伊智朗
  • Organizer: 第35回 日本分子生物学会年会
  • Place of Presentation: 福岡（福岡国際会議場・マリンメッセ福岡）
  • Invited
• [Presentation] Global genome reorganization revealed by genome-wide DNA replication timing analysis: exploring its significance during early stages of mouse development (2011)
  • Author(s): 平谷 伊智朗
  • Organizer: 第34回 日本分子生物学会年会
  • Place of Presentation: 横浜(パシフィコ横浜)
• [Presentation] Global genome reorganization revealed by genome-wide DNA replication timing analysis: exploring its significance during early stages of mouse development
  • Author(s): 平谷伊智朗
  • Organizer: 第34回日本分子生物学会年会(招待講演)
  • Place of Presentation: 横浜（ パシフィコ横浜）