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Study for molecular mechanism of cell proliferation regulation by cell morphology via Hippo signaling pathway

Research Project

Project/Area Number 23770236
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Cell biology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

WADA Ken-ichi  独立行政法人理化学研究所, 前田バイオ工学研究室, 協力研究員 (20525919)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
KeywordsHippo シグナル経路 / 細胞増殖の接触阻止 / 微細加工技術 / 細胞の形 / F-actin / Hippoシグナル経路 / Yap / マイクロドメイン / cell shape / microdomain cell culture / Hippo signal / stress fiber/F-actin / contact inhibition
Research Abstract

In this study, using microdomain cell culture system we investigated molecular mechanism of regulation for cell proliferation by cell morphology via Hippo signaling pathway. We found that cell morphology regulates stress fiber formation and it works as a negative regulator for Hippo signaling pathway. Based on these findings, we proposed a novel model for “contact inhibition of cell proliferation”, in which cell morphology acts as an initial trigger for the regulation for cell proliferation via Hippo signaling pathway by modulation of stress fiber formation.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (12 results)

All 2012 2011 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (4 results) Remarks (6 results)

  • [Journal Article] Hippo pathway regulation by cell morphology and stress fibers.2011

    • Author(s)
      K.-I. Wada, K. Itoga, T. Okano, S. Yonemura, H. Sasaki
    • Journal Title

      Development

      Volume: 138 Pages: 3907-3914

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Modulating F-actin organization induces organ growth by affecting the Hippo pathway.2011

    • Author(s)
      L. Sansores-Garcia, W. Bossuyt, K.-I. Wada, S. Yonemura, C. Tao, H. Sasaki, G. Halder
    • Journal Title

      EMBO Journal

      Volume: 30 Pages: 2325-2335

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] ANALYSIS FOR EFFECT OF CELL SHAPE ON HIPPO SIGNALING PATHWAY USING MICRO-FABRICATED CELL CULTURE PLATFORM2012

    • Author(s)
      Ken-Ichi Wada, Kazuyoshi Itoga, Teruo Okano, Hiroshi Sasaki
    • Organizer
      16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2012)
    • Place of Presentation
      Okinawa, Japan
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Manipulation of Individual Live Cells by Micro-Fabricated Structure2012

    • Author(s)
      Ken-Ichi Wada
    • Organizer
      The 7th Sweden-Japan BioNano Workshop
    • Place of Presentation
      Stockholm, Sweden
    • Related Report
      2012 Annual Research Report
  • [Presentation] Cell Shape and F-actin regulate Yap in parallel to cannonical Hippo Pathway2011

    • Author(s)
      和田健一、糸賀和義、岡野光夫、米村重信、佐々木洋
    • Organizer
      第63回日本細胞生物学会大会
    • Place of Presentation
      北海道大学(北海道)
    • Related Report
      2012 Final Research Report
  • [Presentation] Cell Shape and F-actin Regulate Hippo Signaling Pathway2011

    • Author(s)
      和田健一、糸賀和義、岡野光夫、米村重信、佐々木洋
    • Organizer
      第63回日本細胞生物学会大会
    • Place of Presentation
      北海道札幌市
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.cdb.riken.go.jp/jp/04_news/articles/pdf/11/110915_hipposignaling.pdf

    • Related Report
      2012 Final Research Report
  • [Remarks] 細胞の増殖をコントロールする”かたち”

    • URL

      http://www.cdb.riken.go.jp/jp/04_news/articles/pdf/11/110915_hipposignaling.pdf

    • Related Report
      2012 Annual Research Report
  • [Remarks] 細胞は細胞密度の変化を自分の形で知る

    • URL

      http://www.imeg.kumamoto-u.ac.jp/newpress/np48.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://stke.sciencemag.org/cgi/content/abstract/4/188/ec236

    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.cdb.riken.jp/jp/04_news/articles/11/110915_hipposignaling.html

    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.imeg.kumamoto-u.ac.jp/newpress/np48.html

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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