| Project/Area Number |
23770238
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Aichi Cancer Center Research Institute |
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Principal Investigator |
INOKO Akihito 愛知県がんセンター(研究所), 発がん制御研究部, 主任研究員 (30393127)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 上皮分化 / ケラチン / 中心体 / 細胞周期 / 一次線毛 |
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| Research Abstract |
Keratin cytoskeleton is specifically observed in differentiated epithelial cells. Nevertheless, our knowledge of its contribution to epithelial cell differentiation and proliferation has been fragmentally. We identified keratin filament-binding proteins, Albatross and trichoplein, as TPHD (trichohyalin and plectin homology domain) proteins, according to their unique amino acid sequences. Indeed, growing evidences indicate that TPHD proteins are locationally and functionally "bi-player proteins" which coordinate differentiation and proliferation of epithelia. In this project, we published a report that trichoplein regulates cell proliferation through a novel mechanism, thus, centriolar trichoplein controls primary cilia kinetics that changes cell cycle progression and cellular quiescence. Also, this mechanism raised an idea how primary cilia-deficient cancer cells specifically damaged. Moreover, this finding of G1 phase regulation by primary cilia kinetics allowed us to examine its effect on cell differentiation in the future study.
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