Study on the mechanisms of mitochondrial maintenance by cooperation of proteolysis machineries

• Project/Area Number: 23770239
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: 沖縄科学技術大学院大学
• Principal Investigator: TAKEDA Kojiro 沖縄科学技術大学院大学, グループリーダー (90426578)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 細胞 / 蛋白質 / ミトコンドリア / 蛋白質分解 / 寿命 / タンパク質分解 / プロテアソーム / 細胞周期 / G0期

Research Abstract

In order to identify proteolysis factors, which are involved in maintaining mitochondrial quality, genetic screening was carried out, using Schizosaccharomyces pombe as a model organism. As a result, one potential E3 ubiquitin ligase (RING finger type) was identified to be required for mitochondrial morphology, mitophagy, and maintenance of life span of S.pombe cells in G0/quiescent phase.

Research Products

• [Journal Article] Implications for proteasome nuclear localization revealed by the structure of the nuclear proteasome tether protein Cut8 (2011)
  • Author(s): Takeda, K., Tonthat, N., Glover, T., Xu, W., Koonin, E., Yanagida, M., and Schumacher, M
  • Journal Title: Proc Natl Acad Sci U S A Volume: 108 Pages: 16950-16955
  • Peer Reviewed
• [Journal Article] Identification of genes affecting the toxicity of anti-cancer drug Bortezomib by genome-widescreening in S.pombe (2011)
  • Author(s): Takeda, K., Mori, A., and Yanagida,M.
  • Journal Title: PLoS One Volume: 6(7)
  • Peer Reviewed
• [Journal Article] Mis17 is a regulatory models of the Mis6-Mal2-Sim4 centromere complexthat is required for the recruitment of CenH3/CENP-Ain fissionyeast (2011)
  • Author(s): Shiroiwa, Y., Hayashi, T., Fujita, Y., Villar-Briones, A., Ikai, N., Takeda, K., Ebe, M., and Yanagida,M.
  • Journal Title: PLoS One Volume: 6(3)
  • Peer Reviewed
• [Journal Article] Identification of genes affecting the toxicity of anti-cancer drug bortezomib by genome-wide screening in S.ppombe (2011)
  • Author(s): Kojiro Takeda, Ayaka Mori and Mitsuhiro Yanagida
  • Journal Title: PLoS One Volume: 6 Issue: 7 Pages: 222021-222021
  • DOI: 10.1371/journal.pone.0022021
  • Peer Reviewed
• [Journal Article] Implications for proteasome nuclear localization revealed by the structure of the nuclear proteasome tether protein Cut8 (2011)
  • Author(s): Kojiro Tkeda, Nam K. Tonthat, Tiffany Glover, Weijun Xu, Eugene V. Koonin, Mitsuhiro Yanagida, and Maria A. Schumacher
  • Journal Title: PNAS Volume: 108 Issue: 41 Pages: 16950-16955
  • DOI: 10.1073/pnas.1103617108
  • Peer Reviewed
• [Presentation] 蛋白質分解系の協調によるミトコンドリア品質管理と静止期細胞の寿命維持 (2011)
  • Author(s): 武田鋼二郎
  • Organizer: アステラス病態代謝研究会第42階研究報告会
  • Place of Presentation: 東京
  • Year and Date: 2011-10-15
• [Presentation] The nuclear proteasome tethering factor Cut8 directly binds to the nuclear envelope through 14-3-3-like structure (2011)
  • Author(s): Kojiro Takeda, Nam K tonthat, Tiffany Glover, Weijun Xu, Eugene V. Koonin, Mitsuhiro Yanagida and Maria Schumacher.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] 抗がん剤ボルテゾミブ添加と合成致死となる分裂酵母遺伝子破壊株の単離 (2011)
  • Author(s): 武田鋼二郎,森礼郁,柳田充弘.
  • Organizer: 第44回酵母遺伝学フォーラム
  • Place of Presentation: 福岡
• [Presentation] Implications for proteasome nuclear localization revealed by the structure of the nuclear proteasome tether protein Cut8 (2011)
  • Author(s): Kojiro Takeda, Nam K tonthat, Tiffany Glover, Weijun Xu, Eugene V. Koonin, Mitsuhiro Yanagida and Maria Schumacher.
  • Organizer: The 5th International Workshop on Cell Regulations in Division and Arrest
  • Place of Presentation: Okinawa
• [Presentation] 抗がん剤ボルテゾミブ添加と合成致死となる分裂酵母遺伝子破壊株の単離 (2011)
  • Author(s): 武田鋼二郎
  • Organizer: 酵母遺伝学フォーラム
  • Place of Presentation: 九州大学医学部百年講堂
• [Presentation] 分裂酵母26Sプロテアソーム核／核膜局在化因子Cut8の持つ14-3-3タンパク質様のユニークな構造とその核膜局在化における役割 (2011)
  • Author(s): 武田鋼二郎
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜