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Functional analysis of Nepro, a novel Notch downstream gene requiredfor the maintenance of neocortex neural progenitor cells

Research Project

Project/Area Number 23770242
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Developmental biology
Research InstitutionChiba University

Principal Investigator

MUROYAMA Yuko  千葉大学, 大学院・医学研究院, 講師 (20422248)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords神経幹細胞 / 大脳皮質 / Notch / 発生・分化 / 神経科学 / 幹細胞 / 脳・神経 / 遺伝子
Research Abstract

In a previous study, we identified Nepro, a gene expressed in the developing mouse neocortex at early stages and showed that Nepro is involved in the maintenance of NPCs downstream of Notch. In this study, we examined whether the conserved parts of Nepro are important for its activity by transfecting deletion mutants into NPCs. In contrast to full-length Nepro, the mutant lacking conserved C-terminat part of Nepro did not inhibit neuronal differentiation, suggesting that this region is required for Nepro function. Furthermore, Nepro mutant in which the NLS is disrupted by amino acid substitutions did not exhibit Nepro activity, suggesting that the NLS is important for Nepro function. To search for regulatory regions responsible for Nepro expression in early NPCs, we constructed reporter vectors carrying parts of the Nepro genome. We electroporated them into the embryonic mouse brain and identified a regulatory region located upstream of Nepro, which directs Nepro expression in the early NPCs.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (5 results)

All 2013 2012 2011

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (4 results)

  • [Journal Article] Inducible gene expression in postmitotic neurons by an in vivo electroporation-based tetracycline system2013

    • Author(s)
      Sato, T., Muroyama, Y. and Saito, T
    • Journal Title

      Journal of Neuroscience Methods

      Volume: 214 Issue: 2 Pages: 170-176

    • DOI

      10.1016/j.jneumeth.2013.01.014

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Presentation] Nepro is required for the maintenance of neural progenitor cells in the early neocortex2012

    • Author(s)
      Yuko Muroyama, Tatsuya Sato and Tetsuichiro Saito
    • Organizer
      1st International Symposium "Neocortical Organization"
    • Place of Presentation
      岡崎
    • Related Report
      2012 Final Research Report
  • [Presentation] Nepro is required for the maintenance of neural stem cells in the early neocortex.2012

    • Author(s)
      斎藤哲一郎
    • Organizer
      1st International Symposium "Neocortical Organization"
    • Place of Presentation
      岡崎カンファレンスセンター(愛知県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Functional analysis of Nepro, a gene required for the maintenance of neocortex neural progenitor cells2011

    • Author(s)
      Tatsuya Sato, Yuko Muroyama and Tetsuichiro Saito
    • Organizer
      第34回日本神経科学会大会
    • Place of Presentation
      横浜
    • Related Report
      2012 Final Research Report
  • [Presentation] 大脳皮質神経前駆細胞の維持に必要な遺伝子Neproの機能解析2011

    • Author(s)
      Tatsuya Sato
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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