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Functional analysis of the PFR during digit development

Research Project

Project/Area Number 23770244
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Developmental biology
Research InstitutionNagoya University

Principal Investigator

SUZUKI Takayuki  名古屋大学, 理学(系)研究科(研究院), 助教 (40451629)

Co-Investigator(Renkei-kenkyūsha) MORISHITA Yoshihiro  理化学研究所, 発生再生科学総合研究センター, ユニットリーダー (00404062)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords肢芽 / 指 / 指間部 / 画像解析 / 定量化 / シミュレーション / 数理生物学 / モデル / 変形 / 発生 / 指の個性 / BMP / ニワトリ / PFR / 形態形成 / シグナル伝達 / 軟骨
Research Abstract

We found that the cells located distal to the digital lay received positional information from inter digit. These cells get BMP signaling from adjacent interdigit located at the posterior side. I named this region PFR. Surprisingly, PFR cells have unidirectional sensitivity which means PFR cells respond to BMP signaling coming from only posterior interdigit. In this study, we focused on the origin of the interdigit that sends positional information to the PFR. We have developed a novel method that combines snapshot lineage tracing with Bayesian statistical estimation to construct whole-organ deformation maps from landmark data on limited numbers of space-time points. We applied it to chick limb development. A quantitative tissue deformation map for St.23 and St.24 has been constructed, and its precision has been proven by evaluating its predictive performance. Thus, our method enables deformation dynamics analysis in organogenesis using practical lineage marking techniques.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (22 results)

All 2014 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (12 results) (of which Invited: 2 results) Book (1 results) Remarks (4 results)

  • [Journal Article] Bayesian inference of whole-organ deformation dynamics from limited space-time point data2014

    • Author(s)
      Morishita Y. and Suzuki T.
    • Journal Title

      J. Thel. Biol

      Volume: 14

    • Related Report
      2013 Final Research Report
  • [Journal Article] Reconstruction and in vivo analysis of the extinct tbx5 gene from ancient wingless moa (Aves : Dinornithiformes)2014

    • Author(s)
      Huynen L., Suzuki T. Ogura T. Watanabe Y. Millar C. D. Hofreiter M., Smith C. Mirmoeini S. and Lambert D. M.
    • Journal Title

      BMC Evolutionary Biology

      Volume: 14

    • Related Report
      2013 Final Research Report
  • [Journal Article] Bayesian inference of whole-organ deformation dynamics from limited space-time point data2014

    • Author(s)
      Y. Morishita and T. Suzuki
    • Journal Title

      J Theor Biol

      Volume: 357 Pages: 74-85

    • DOI

      10.1016/j.jtbi.2014.04.027

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] How is digit identity determined during limb development?2013

    • Author(s)
      Takayuki Suzuki
    • Journal Title

      Development Growth and Differentiation

      Volume: 55 Issue: 1 Pages: 130-138

    • DOI

      10.1111/dgd.12022

    • NAID

      40019549295

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of spontaneous mutations within the long-range limb-specific Sonic hedgehog enhancer (ZRS) that alter Sonic hedgehog expression in the chicken limb mutants oligozeugodactyly and silkie breed2011

    • Author(s)
      Maas S.A.*, Suzuki T.*, Fallon J.F.*Equal contribution
    • Journal Title

      Developmental Dynamics

      Volume: 240 Issue: 5 Pages: 1212-1212

    • DOI

      10.1002/dvdy.22634

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 劣性遺伝で多指症を発症するHMM変異体の発生学的解析2013

    • Author(s)
      鈴木孝幸、中野幹治、都築政起、松田洋一、黒岩厚
    • Organizer
      第1回ウズラ研究集会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2013-12-10
    • Related Report
      2013 Final Research Report
  • [Presentation] Gdf11の発現開始タイミングの違いが脊椎動物における後肢の位置を決定する2013

    • Author(s)
      Takayuki Suzuki, Yoshiyuki Matsubara, Ayumi Hattori, Toshihiko Ogura, Se-Jin Lee, Atsushi Kuroiwa
    • Organizer
      数理生物学サマーレクチャーコース
    • Place of Presentation
      理化学研究所
    • Year and Date
      2013-07-30
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Expression timing of Gdf11 reveals positional diversity of the hindlimb in vertebrates2013

    • Author(s)
      Takayuki Suzuki, Yoshiyuki Matsubara, Ayumi Hattori, Toshihiko Ogura, Se-Jin Lee, Atsushi Kuroiwa
    • Organizer
      17th International Congress of Developmental Biology
    • Place of Presentation
      Cancun, Mexico
    • Year and Date
      2013-06-14
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Quantitative approach to understand whole organ morphogenesis2013

    • Author(s)
      Takayuki Suzuki
    • Organizer
      第46回日本発生生物学会大会
    • Place of Presentation
      くにびきメッセ
    • Year and Date
      2013-05-28
    • Related Report
      2013 Final Research Report
  • [Presentation] Quantitative approach to understand whole organ morphogenesis2013

    • Author(s)
      Takayuki Suzuki
    • Organizer
      第46回日本発生生物学会大会
    • Place of Presentation
      くにびきメッセ
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 劣性遺伝で多指症を発症するHMM変異体の発生学的解析2013

    • Author(s)
      鈴木孝幸、中野幹治、都築政起、松田洋一、黒岩厚
    • Organizer
      第1回ウズラ研究集会
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 器官全体の形態形成を明らかにするために必要な特徴量の提案と定量解析2013

    • Author(s)
      鈴木孝幸
    • Organizer
      新学術領域第3回若手の会
    • Place of Presentation
      ラフォーレ那須、栃木
    • Related Report
      2013 Annual Research Report
  • [Presentation] Mechanisms of the PFR that responds to only posterior interdigits in the chick limb2012

    • Author(s)
      Takayuki Suzuki
    • Organizer
      45th Annual Meeting of the Japanese Society of Developmental Biologists
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Related Report
      2012 Research-status Report
  • [Presentation] Quantification and visualization of morphogen activity in the developing chick limb bud2012

    • Author(s)
      Takayuki Suzuki
    • Organizer
      7th International Chick meeting
    • Place of Presentation
      名古屋大学野寄記念学術交流館(愛知)
    • Related Report
      2012 Research-status Report
  • [Presentation] 発光顕微鏡を用いた肢芽におけるMorphogenシグナルの定量化と形態形成2012

    • Author(s)
      鈴木孝幸
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Expression timing of Gdf11 determines hindlimb position in vertebrates2012

    • Author(s)
      Takayuki Suzuki
    • Organizer
      12th International conference on Development and Regeneration
    • Place of Presentation
      Mont-tremblant (Quebec, Canada)
    • Related Report
      2012 Research-status Report
  • [Presentation] Quantification and visualization of dynamic SHH signaling activity in the developing chick limb bud2011

    • Author(s)
      Takayuki Suzuki
    • Organizer
      第6回国際ニワトリ学術集会
    • Place of Presentation
      ロスリン(イギリス)
    • Related Report
      2011 Research-status Report
  • [Book] SecondarySmad1/5/8-Dependent Signaling Downstream of SHH Determines Digit Identity2014

    • Author(s)
      鈴木孝幸
    • Publisher
      Springer
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Remarks]

    • URL

      http://bunshi5-bio-nagoya-u.businesscatalyst.com

    • Related Report
      2013 Final Research Report
  • [Remarks] 名古屋大学形態発生学グループ

    • URL

      http://bunshi5-bio-nagoya-u.businesscatalyst.com/index.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] Developmental Morphogenesis

    • URL

      http://bunshi5-bio-nagoya-u.businesscatalyst.com/index.html

    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://web.me.com/stakayuki77/Takayuki/Takayuki_Suzuki.html

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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