| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
The purpose of this project is to analyze molecular mechanism of boundary formation between cells. Zebrafish stripe consists of two types of pigment cells, melanophores and xanthophores. We analyzed pigment pattern mutant, dali, which shows intermingled pigment pattern of melanophores and xanthophores. We identified an amino acid substitution in Tetraspanin 3C (Tespan3C) protein which has a function to anchor membrane proteins at cell surface. The mutation caused defects in protein modification of Tespan3C and in cell migration activity of melanophore. Furthermore we detected the loss of repulsive interaction between melanophore and xanthophore, which caused intermingled pigment pattern of the mutant fish.
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