| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
The cyclostome lampreys are a group of vertebrates that diverged early in evolution and retain various ancestral morphological traits. In order to explore the developmental mechanisms underlying the establishment of germ cell lineage, we examined the emergence of primordial germ cells (PGCs) and the gonads in the embryonic lampreys. Genes involved in the specification of PGCs, migration of PGCs to the gonads and in the establishment of gonadal primordium were newly identified by RT-PCR. Expression of each gene was analyzed by whole mount in situ hybridization. The results showed that maternal mRNAs of several germ cell-related genes were localized in fertilized eggs of the lamprey. We also established the experimental system in which migratory cells in the early embryos can be traced with the expression of the reporter fluorescent protein driven by upstream regulatory sequence of CXCR4 gene.
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