Screening and identification of differentially expressed genes in gonadal tissues in colonial ascidian
| Project/Area Number |
23770253
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Kochi University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 生殖細胞 / 配偶子形成 / 幹細胞 / 生殖系列幹細胞 / 配偶子幹細胞 / ホヤ / 群体ホヤ / 発生 / 遺伝子 |
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| Research Abstract |
Animals that propagate asexually are exciting models to investigate the cellular system, which produces germline cells constitutively throughout life. In botryllid ascidians, each of the asexual individuals produces hermaphrodite gonads during breading season. To understand the molecular mechanisms underlying gamete and gonadal development, screening and identification of differentially expressed genes in gonadal tissues were performed. Subtractive cDNA library was constructed from cDNAs synthesized from the Botryllus primigenus colonies with and without mature gonads. 104 transcripts were isolated from the library. We then determined the ratio of the expression in colonies with mature gonads to the expression in asexually growing colonies by qRT-PCR. Among the transcripts, 91 showed higher ratios above 1.. In order to elucidate the roles of the genes chosen from the list, functional analyses with genetic knockdown using siRNAs have been successfully performed.
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Report
(4 results)
Research Products
(16 results)
