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Screening and identification of differentially expressed genes in gonadal tissues in colonial ascidian

Research Project

Project/Area Number 23770253
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Developmental biology
Research InstitutionKochi University

Principal Investigator

SUNANAGA Takeshi  高知大学, 教育研究部自然科学系, 講師 (20448393)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords生殖細胞 / 配偶子形成 / 幹細胞 / 生殖系列幹細胞 / 配偶子幹細胞 / ホヤ / 群体ホヤ / 発生 / 遺伝子
Research Abstract

Animals that propagate asexually are exciting models to investigate the cellular system, which produces germline cells constitutively throughout life. In botryllid ascidians, each of the asexual individuals produces hermaphrodite gonads during breading season. To understand the molecular mechanisms underlying gamete and gonadal development, screening and identification of differentially expressed genes in gonadal tissues were performed. Subtractive cDNA library was constructed from cDNAs synthesized from the Botryllus primigenus colonies with and without mature gonads. 104 transcripts were isolated from the library. We then determined the ratio of the expression in colonies with mature gonads to the expression in asexually growing colonies by qRT-PCR. Among the transcripts, 91 showed higher ratios above 1.. In order to elucidate the roles of the genes chosen from the list, functional analyses with genetic knockdown using siRNAs have been successfully performed.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (16 results)

All 2013 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (10 results) Remarks (2 results)

  • [Journal Article] Senescence-associated superoxide dismutase influences mitochondrial gene expression in budding tunicates.2013

    • Author(s)
      Kawamura, K. and Sunanaga, T.
    • Journal Title

      Development Growth & Differentiation

      Volume: 55 Issue: 5 Pages: 606-614

    • DOI

      10.1111/dgd.12065

    • NAID

      40019735147

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular anatomy of tunicate senescence: Reversible function of mitochondrial and nuclear genes associated with budding cycles2012

    • Author(s)
      Kawamura K., Kitamura S., Sekida S., Tsuda M., and Sunanaga T
    • Journal Title

      Development

      Volume: 139 Issue: 21 Pages: 4083-4093

    • DOI

      10.1242/dev.083170

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] RACK1 regulates mesenchymal cell recruitment during sexual and asexual reproduction of budding tunicates2012

    • Author(s)
      Tatzuke, Y., Sunanaga, T., Fujiwara, S.& Kawamura, K.
    • Journal Title

      Dev. Biol

      Volume: Vol. 368 Issue: 2 Pages: 393-403

    • DOI

      10.1016/j.ydbio.2012.06.006

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Role of Vasa, Piwi, and Myc-expressing cells in gonad regeneration of the colonial tunicate, Botryllus primigenus2011

    • Author(s)
      Kawamura, K., Sunanaga, T.
    • Journal Title

      Mech.Dev.

      Volume: 128 Issue: 7-10 Pages: 457-470

    • DOI

      10.1016/j.mod.2011.09.001

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 群体ホヤの生殖腺形成に関わる遺伝子群の単離2013

    • Author(s)
      砂長毅,大月恵,黒田紗希,川村和夫
    • Organizer
      日本動物学会84回大会
    • Place of Presentation
      岡山市
    • Year and Date
      2013-09-28
    • Related Report
      2013 Final Research Report
  • [Presentation] Screening and identification of differentially expressed genes in gonadal tissues in colonial ascidian, Botryllus primigenus2013

    • Author(s)
      Sunanaga, T., Kuroda, S., Otsuki, M. and Kawamura, K.
    • Organizer
      7^<th> TUNICATE MEETING
    • Place of Presentation
      ナポリ
    • Year and Date
      2013-07-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Screening and identification of differentially expressed genes in gonadal tissues in colonial ascidian, Botryllus primigenus.2013

    • Author(s)
      Sunanaga, T., Kuroda, S., Otsuki, M. and Kawamura, K.
    • Organizer
      7th TUNICATE MEETING
    • Place of Presentation
      Parthenope University of Naples, Naples, Italy
    • Related Report
      2013 Annual Research Report
  • [Presentation] 群体ホヤの生殖腺形成に関わる遺伝子群の単離2013

    • Author(s)
      砂長毅・大月恵・黒田紗希・川村和夫
    • Organizer
      日本動物学会84回大会
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Cellular and Molecular Basis for Germline Specification in Colonial Ascidian, Botryllus primigenus2012

    • Author(s)
      Sunanaga, T. and Kawamura, K.
    • Organizer
      Asia-Pacific Developmental Biology Conference
    • Place of Presentation
      台北市
    • Year and Date
      2012-10-06
    • Related Report
      2013 Final Research Report
  • [Presentation] Isolation and spatiotemporal expression analysis of BMP in the Botryllus primigenus2012

    • Author(s)
      Ryuzaki, M., Kawamura, K. and Sunanaga, T.
    • Organizer
      日本発生生物学会第45回大会
    • Place of Presentation
      神戸市
    • Year and Date
      2012-05-31
    • Related Report
      2013 Final Research Report
  • [Presentation] Cellular and Molecular Basis for Germline Specification in Colonial Ascidian, Botryllus primigenus2012

    • Author(s)
      Sunanaga, T. and Kawamura, K.
    • Organizer
      58th/60th NIBB conference
    • Place of Presentation
      岡崎市
    • Related Report
      2013 Final Research Report
  • [Presentation] Isolation and spatiotemporal expression analysis of BMP in the Botryllus primigenus2012

    • Author(s)
      Ryuzaki, M., Kawamura, K. and Sunanaga, T.
    • Organizer
      日本発生生物学会第45回大会
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Related Report
      2012 Research-status Report
  • [Presentation] Cellular and Molecular Basis for Germline Specification in Colonial Ascidian, Botryllus primigenus2012

    • Author(s)
      Sunanaga, T. and Kawamura, K.
    • Organizer
      Asia-Pacific Developmental Biology Conference 2012
    • Place of Presentation
      Taipei Innovation City Convention Center(台湾台北市 )
    • Related Report
      2012 Research-status Report
  • [Presentation] Cellular and Molecular Basis for Germline Specification in Colonial Ascidian, Botryllus primigenus2012

    • Author(s)
      Sunanaga, T. and Kawamura, K.
    • Organizer
      58th/60th NIBB conference
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県岡崎市)
    • Related Report
      2012 Research-status Report
  • [Remarks] 砂長 毅 「幹細胞を上手に使って増やす,治す」 高知県鍼灸師会学術研修会 高知市 高知共済会館 平成25年2月17日 講演活動

    • Related Report
      2013 Final Research Report
  • [Remarks] 砂長 毅 「幹細胞を上手に使って増やす,治す」 高知県鍼灸師会学術研修会 高知市 高知共済会館 平成23年10月23日 講演活動

    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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