| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Research Abstract |
We performed transcriptome analysis with mouse epiblasts, which were isolated from the E5.5 mouse embryos cultured for short time in the presence or absence of a chemical inhibitor specific to Nodal signaling. As the result, we identified a number of direct target genes whose expression level was altered by the inhibitor. In particular, genes upregulated by the inhibitor included those involved in neural fate decision. We further validated the transcriptome analyses with in situ hybridization method for genes involved in mouse early development. Therefore, we could list the direct target genes of Nodal signaling out of those already known to be altered in Nodal knock-out mice.
|
