Research of transcriptional repression mechanism by histone acetylation in the maintenance of cell fate

• Project/Area Number: 23770257
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Kwansei Gakuin University
• Principal Investigator: SHIBATA Yukimasa 関西学院大学, 理工学研究科, 博士研究員 (80314053)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: クロマチン / BET / 細胞運命維持 / UTX / H2A.z / アセチル化 / 細胞運命の維持

Research Abstract

Histone acetylation is required for the maintenance of cell fates. But, its molecular mechanism is largely unknown. In this study, we investigate the dynamics of chromatin status that regulate cell-fate maintenance. As a results, we found that acetylated histone recruit histone variant HTZ-1/H2A.z on the loci that encodes transcription factor, and repress the locus. The recruitment of HTZ-1 is inhibited by H3K27me. We also found that the CeBAF complex that control histone deposition is required for the maintenance of cell fates.

Research Products

• [Journal Article] HTZ-1/H2A.z and MYS-1/MYST HAT act redundantly to maintain cell fates in somatic gonadal cells through repression of ceh-22 in Caenorhabditis elegans. (2014)
  • Author(s): Shibata Y, Sawa H, Nishiwaki K
  • Journal Title: Development Volume: 141(1) Issue: 1 Pages: 209-218
  • DOI: 10.1242/dev.090746
  • Peer Reviewed
• [Journal Article] The novel secreted factor MIG-18 acts with MIG-17/ADAMTS to control cell migration in Caenorhabditis elegans. (2014)
  • Author(s): Kim H-S, Kitano Y, Mori M, Takano T, Harbaugh TE, Mizutani K, Yanagimoto H, Miwa S, Ihara S, Kubota Y, Shibata Y, Ikenishi K, Garriga G and Nishiwaki K.
  • Journal Title: Genetics Volume: 196(2) Issue: 2 Pages: 471-479
  • DOI: 10.1534/genetics.113.157685
  • URL: http://www.genetics.org/content/196/2/471.long
  • Peer Reviewed / Open Access
• [Journal Article] Multiple functions of PBRM-1/Polybromo- and LET-526/Osa-containing chromatin remodeling complexes in C.elegans development (2011)
  • Author(s): Shibata Y, Uchida M, Takeshita H, Nishiwaki K., Sawa H
  • Journal Title: Dev Biol Volume: 361 Issue: 2 Pages: 349-357
  • DOI: 10.1016/j.ydbio.2011.10.035
  • Peer Reviewed
• [Presentation] HTZ-1/H2A.z maintains cell fates through transcriptional repression in an H3K27me-independent manner (2013)
  • Author(s): Yukimasa Shibata, Hitoshi Sawa, Kiyoji Nishiwaki
  • Organizer: 19th International C. elegans Meeting
  • Place of Presentation: University of California (Los Angeles)
  • Year and Date: 2013-06-27
• [Presentation] HTZ-1/H2A.z maintains cell fates through transcriptional repression in an H3K27me-independent manner (2012)
  • Author(s): Yukimasa Shibata, Hitoshi Sawa, Kiyoji Nishiwaki
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場、福岡県
  • Year and Date: 2012-12-13
• [Presentation] HTZ-1/H2A.z maintains the fates of somatic gonadal cells through the repression of ceh-22/Hox in an H3K27me-independent manner (2012)
  • Author(s): Yukimasa Shibata, Hitoshi Sawa, Kiyoji Nishiwaki
  • Organizer: 5th East Asia C. elegans Meeting
  • Place of Presentation: Chientan Youth Activity Center、台湾
  • Year and Date: 2012-06-29
• [Presentation] HTZ-1/H2A.z maintains the fates of somatic gonadal cells through the repression of ceh-22/Hox in an H3K27me-independent manner (2012)
  • Author(s): Yukimasa Shibata, Hitoshi Sawa, Kiyoji Nishiwaki
  • Organizer: Joint meeting of JSDB 45th and JSCB 64^<th>
  • Place of Presentation: 神戸国際会議場、神戸市
  • Year and Date: 2012-05-31
• [Presentation] C. elegans H2A.zはホメオボックス遺伝子ceh-22の発現抑制を介して生殖巣リーダー細胞運命を抑制する (2011)
  • Author(s): 柴田幸政、澤斉、西脇清二
  • Organizer: 第34回分子生物学会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2011-12-15
• [Presentation] The histone acetylation, methylation, and H2A.z are involved in the maintenance of cell fates in C. elegans (2011)
  • Author(s): Yukimasa Shibata, Hitoshi Sawa, Kiyoji Nishiwaki
  • Organizer: 18^<th> International C. elegans Meeting
  • Place of Presentation: University of California (Los Angeles)
  • Year and Date: 2011-06-24
• [Presentation] 細胞運命の維持における、アセチル化ヒストン結合蛋白BET-1とヒストンバリアントH2A,.zによる転写調節 (2011)
  • Author(s): 柴田幸政、澤斉、西脇清二
  • Organizer: 第44回発生生物学会
  • Place of Presentation: 沖縄コンベンションセンター(沖縄)
  • Year and Date: 2011-05-20
• [Presentation] 細胞運命の維持における、アセチル化ヒストン結合蛋白BET-1とヒストンバリアントH2A,.zによる転写調節 (2011)
  • Author(s): 柴田 幸政、澤 斉、西脇 清二
  • Organizer: 第４４回発生生物学会
  • Place of Presentation: 沖縄コンベンションセンター(沖縄）
• [Presentation] The histone acetylation, methylation, and H2A.z are involved in the maintenance of cell fates in C. elegans (2011)
  • Author(s): Yukimasa Shibata, Hitoshi Sawa, Kiyoji Nishiwaki
  • Organizer: 18th International C. elegans Meeting
  • Place of Presentation: University of California (Los Angeles)
• [Presentation] C. elegans H2A.zはホメオボックス遺伝子ceh-22の発現抑制を介して生殖巣リーダー細胞運命を抑制する (2011)
  • Author(s): 柴田 幸政、澤 斉、西脇 清二
  • Organizer: 第３４回分子生物学会
  • Place of Presentation: パシフィコ横浜(横浜）
• [Presentation] HTZ-1/H2A.z maintains cell fates through transcriptional repression in an H3K27me-independent manner
  • Author(s): Yukimasa Shibata, Hitoshi Sawa, Kiyoji Nishiwaki
  • Organizer: 19th International C. elegans Meeting
  • Place of Presentation: University of California, Los Angels, USA
• [Presentation] HTZ-1/H2A.z maintains the fates of somatic gonadal cells through the repression of ceh-22/Hox in an H3K27me-independent manner
  • Author(s): Yukimasa Shibata, Hitoshi Sawa, Kiyoji Nishiwaki
  • Organizer: Joint meeting of JSDB 45th and JSCB 64th
  • Place of Presentation: 神戸国際会議場、神戸市