Evolutionary utilization of DNA methylation for regulation of gene expression in primitive vertebrates
| Project/Area Number |
23770273
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Evolutionary biology
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| Research Institution | National Research Institute for Child Health and Development (2012-2014)
Ochanomizu University (2011) |
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Principal Investigator |
OKAMURA Kohji 独立行政法人国立成育医療研究センター, システム発生・再生医学研究部, 室長 (80456194)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | DNAメチル化 / エピジェネティクス / 遺伝子発現 / 原索動物 / 転写開始点 / プロモータ / CpGアイランド |
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| Outline of Final Research Achievements |
Vertebrates and invertebrates differ not only in the possession of a backbone, but also in the regulation of gene expression. Unlike in mice and humans, DNA methylation is not considered to be crucial for gene transcription in invertebrates. Accordingly, some organisms including larvaceans, fruit flies, nematodes, and yeasts have lost the CpG methylation system, whereas many prokaryotes still retain it. In the present study, we focused on the lamprey and ascidian―organisms regarded as evolutionary links between vertebrates and invertebrates―and examined the genomic sequences of their promoters. Total RNA samples were extracted from whole embryos to cover many promoters, and their sequences were determined by RNA-seq. Genome-wide DNA methylation analysis was also performed at base resolution using the PBAT method for both species. As a result, two types of promoters were clearly characterized in terms of the use of DNA methylation for each transcriptional system.
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Report
(5 results)
Research Products
(35 results)
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[Journal Article] DNA methylation analysis of human myoblasts during in vitro myogenic differentiation: de novo methylation of promoters of muscle-related genes and its involvement in transcriptional down-regulation2015
Author(s)
Miyata K, Miyata T, Nakabayashi K, Okamura K, Naito M, Kawai T, Takada S, Kato K, Miyamoto S, Hata K, Asahara H
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Journal Title
Hum. Mol. Genet.
Volume: 24
Issue: 2
Pages: 410-423
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Genome-wide parent-of-origin DNA methylation analysis reveals the intricacies of the human imprintome and suggests a germline methylation independent establishment of imprinting.2014
Author(s)
Court F, Tayama C, Romanelli V, Martin-Trujillo A, Iglesias-Platas I, Okamura K, Sugahara N, Simon C, Moore H, Harness J, Keirstead H, Vicente Sanchez-Mut J, Kaneki E, Lapunzina P, Soejima H, Wake N, Esteller M, Ogata T, Hata K, Nakabayashi K, Monk D*
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Journal Title
Genome Res
Volume: in press
Issue: 4
Pages: 554-569
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Compilation of copy number variants identified in phenotypically normal and parous Japanese women2014
Author(s)
Migita O, Maehara K, Kamura H, Miyakoshi K, Tanaka M, Morokuma S, Fukushima K, Shimamoto T, Saito S, Sago H, Nishihama K, Abe K, Nakabayashi K, Umezawa A, Okamura K, Hata K
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Journal Title
J. Hum. Genet.
Volume: 59
Issue: 6
Pages: 326-331
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Ataxia telangiectasia derived iPS cells show preserved x-ray sensitivity and decreased chromosomal instability2014
Author(s)
Fukawatase Y, Toyoda M, Okamura K, Nakamura K, Nakabayashi K, Takada S, Yamazaki-Inoue M, Masuda A, Nasu M, Hata K, Hanaoka K, Higuchi A, Takubo K, Umezawa A
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Journal Title
Sci. Rep.
Volume: 4
Issue: 1
Pages: 5421-5421
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Characterization of the compact bicistronic microRNA precursor, miR-1/miR-133, expressed specifically in Ciona muscle tissues.2013
Author(s)
Rie Kusakabe, Saori Tani, Koki Nishitsuji, Miyuki Shindo, Kohji Okamura, Yuki Miyamoto, Kenta Nakai, Yutaka Suzuki, Takehiro G Kusakabe, Kunio Inoue
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Journal Title
Gene Expression Patterns
Volume: 13
Issue: 1-2
Pages: 43-50
DOI
Related Report
Peer Reviewed
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[Presentation] ヒト老化細胞を利用したエピゲノム解析2014
Author(s)
榊 みずほ, 老原 侑子, 岡村 浩司, 中林 一彦, 秦 健一郎, 小林 芳郎, 前原 佳代子
Organizer
第37回日本分子生物学会年会
Place of Presentation
パシフィコ横浜
Year and Date
2014-11-27
Related Report
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[Presentation] Copy number variants identified in Japanese women2014
Author(s)
Ohsuke Migita, Kayoko Maehara, Kazuhiko Nakabayashi, Kohji Okamura, and Kenichiro Hata
Organizer
The 64th Annual Meeting of the American Society of Human Genetics
Place of Presentation
San Diego Convention Center, San Diego, U. S.
Year and Date
2014-10-20
Related Report
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[Presentation] 子宮平滑筋肉腫の分子病態の統合解析2013
Author(s)
宮田 知子, 中林 一彦, 岡村 浩司, 小林 裕明, 奥川 馨, 矢幡 秀昭, 園田 顕三, 加藤 聖子, 秦 健一郎
Organizer
第7回日本エピジェネティクス研究会年会 P-16
Place of Presentation
奈良県新公会堂
Related Report
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