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Studies of the diversity and molecular evolution of defensive mechanism in termites

Research Project

Project/Area Number 23770274
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Evolutionary biology
Research InstitutionUniversity of the Ryukyus

Principal Investigator

HOJO Masaru  琉球大学, 熱帯生物圏研究センター, 研究員 (80569898)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords機能進化 / Distal-less / ジテルペン合成 / ゲラニルゲラニル二リン酸 / RNA-Seq / 次世代シークエンサー / 発現解析 / ゲラニルゲラニル二リン酸合成酵素 / テルペノイド / 化学的防衛 / カースト分化 / シロアリ / ヒドロキシメチルグルタリルCoA / HMG-CoA合成酵素 / HMG-CoAレダクターゼ / RNA干渉
Research Abstract

To clarify the diversity and evolutional mechanisms of termite defensive system, I searched for the genes involved with the development of horn-like frontal projection (nasus) and the synthesis of cembrene-derived polycyclic diterpenes by using Nasutitermes takasagoensis which is the most derived termite species. The results indicated that a homeobox gene Distal-less is related to the development of the nasus. Moreover, all genes involved with the precursor of diterpene were determined using next generation sequencer. Especially, geranylgeranyl diphosphate synthase genes were duplicated repeatedly. These results suggest that the synthesis of diterpene is to contribute to the acquisition of a novel defensive system in a termite lineage, coupled with the acquisition of adaptive defensive behaviors.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (12 results)

All 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 3 results) Presentation (5 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Exploration and characterization of genes involved in the synthesis of diterpene defense secretion in nasute termite soldiers2012

    • Author(s)
      M. Hojo, K. Maekawa, S. Saitoh, S. Shigenobu, T. Miura, Y. Hayashi, G. Tokuda, H. Maekawa
    • Journal Title

      Insect Molecular Biology

      Volume: 21 Pages: 545-557

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression and function of a limb-patterning gene distal-less in the soldier-specific morphogenesis in the nasute termite Nasutitermes takasagoensis2012

    • Author(s)
      K. Toga, M. Hojo, T. Miura, K. Maekawa
    • Journal Title

      Evolution and Devlopment

      Volume: 14 Pages: 286-295

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cellulolytic environment in the midgut of the wood-feeding higher termite Nasutitermes takasagoensis2012

    • Author(s)
      Gaku Tokuda, Hirofumi Watanabe, Masaru Hojo, Ai Fujita, Hiromi Makiya, Mio Miyagi, Gaku Arakawa, Manabu Arioka
    • Journal Title

      Journal of Insect Physiology

      Volume: 58 Pages: 147-154

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 日本のシロアリの様々な防衛方法2011

    • Author(s)
      北條優
    • Journal Title

      しろあり

      Volume: 156 Pages: 19-27

    • NAID

      40018956135

    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] タカサゴシロアリにおけるトランスクリプトーム解析から見えてきた攻撃方法の進化2012

    • Author(s)
      北條優
    • Organizer
      日本昆虫学会第72回大会
    • Place of Presentation
      玉川大学
    • Year and Date
      2012-09-16
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Exploration and characterization of genes involved in the diterpene synthetic pathway for defensive secretion in nasute termite soldiers2012

    • Author(s)
      M. Hojo
    • Organizer
      XXIV International Congress of Entomology
    • Place of Presentation
      Daegu, Korea
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] 高等シロアリ中腸環境におけるセルロース消化系2012

    • Author(s)
      徳田岳、北條優、渡辺裕文
    • Organizer
      日本蚕糸学会「平成24年度蚕糸・昆虫機能利用学術講演会」
    • Place of Presentation
      九州大学箱崎キャンパス
    • Related Report
      2011 Research-status Report
  • [Presentation] タカサゴシロアリ中腸における消化酵素の分布様式と特性2012

    • Author(s)
      徳田岳、北條優、仲原宏美、渡辺裕文、有岡学
    • Organizer
      第56回日本応用動物昆虫学会大会
    • Place of Presentation
      近畿大学農学部奈良キャンパス
    • Related Report
      2011 Research-status Report
  • [Presentation] タカサゴシロアリの兵隊におけるテルペン合成遺伝子の発現解析2011

    • Author(s)
      北條優
    • Organizer
      日本昆虫学会第71回大会
    • Place of Presentation
      信州大学
    • Year and Date
      2011-09-19
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Book] シロアリの事典-4.4. テングシロアリの兵隊におけるジテルペンの合成2012

    • Author(s)
      吉村剛(編)、北條優
    • Publisher
      海青社
    • Related Report
      2012 Final Research Report
  • [Book] シロアリの事典 「4.4 テングシロアリの兵隊におけるジテルペンの合成」2012

    • Author(s)
      北條優
    • Total Pages
      472
    • Publisher
      海青社
    • Related Report
      2012 Annual Research Report
  • [Remarks] 平成24年度熱帯生物圏研究センター市民公開講座・展示.「知られざる兵隊シロアリの攻撃方法」沖縄県立博物館・美術館.2012 年9月2 日

    • Related Report
      2012 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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