Comprehensive genetic evaluation of tomato fruit expansion by the breeding use of mutant bioresources

• Project/Area Number: 23780001
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Breeding science
• Research Institution: University of Tsukuba
• Principal Investigator: ARIIZUMI Ariizumi 筑波大学, 生命環境系, 助教 (70575381)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: トマト / 変異体 / 果実肥大 / 交配育種 / 果実肥大性 / 単為結果性 / 植物遺伝育種

Research Abstract

This research explores the possibility whether addition of different mutations associated with fruit size results in increased fruit expansion (subject 1), and aims to isolate the responsible gene for one of the fruit size mutant (subject 2). Genetic analysis indicated double mutants showedincreased fruit expansion, consistent with our prediction. In this research, genome sequences of two out of four mutants were determined by next generation sequencing and it appeared that these two genes encode novel proteins that control fruit size.

Research Products

• [Journal Article] Genes that influence yield in tomato (2013)
  • Author(s): Ariizumi T, Shinozaki Y, Ezura H
  • Journal Title: Breeding Science Volume: 63 Issue: 1 Pages: 3-13
  • DOI: 10.1270/jsbbs.63.3
  • NAID: 10031151519
  • ISSN: 1344-7610, 1347-3735
• [Presentation] Characterization of tomato bubble fruit mutants which form bubble-like fruit structure at the tip of fruit (2013)
  • Author(s): Kimura A, Ariizumi T, Okabe Y, Saito T, Asamizu E, Sakakibara H, Kojima M, Ezura H
  • Organizer: 第54回日本植物生理学会
  • Place of Presentation: 岡山大学
• [Presentation] Analysis of effect of the bubble fruit mutation on pistil development, ploidy levels, and fruit structure in tomato (2013)
  • Author(s): Kimura A, Ariizumi T, Okabe Y, Saito T, Asamizu E, Sakakibara S, Kojima M, Ezura H
  • Organizer: International Symposium on Japanese Solanaceae Genomics Initiative (JSOL)
  • Place of Presentation: 筑波大学
• [Presentation] RNA-seq based Transcript Profiling of Auxin-dependent Fruit Set in Tomato (2013)
  • Author(s): Shinozaki Y, Hao S, Fei Z, Zhong S, Giovanonni J, Rose JK, Ezura H, Ariizumi T
  • Organizer: International Symposium on Japanese Solanaceae Genomics Initiative (JSOL) 2013
  • Place of Presentation: 筑波大学
• [Presentation] Characterization of tomato bubble fruit mutants which form bubble-like fruit structure at the tip of fruit. (2013)
  • Author(s): Kimura A, Ariizumi T, Okabe Y, Saito T, Asamizu E, Sakakibara H, Kojima M, Ezura H
  • Organizer: 第54回日本植物生理学会
  • Place of Presentation: 岡山大学
• [Presentation] Analysis of effect of the bubble fruit mutation on pistil development, ploidy levels, and fruit structure in tomato. (2013)
  • Author(s): Kimura A, Ariizumi T, Okabe Y, Saito T, Asamizu E, Sakakibara S, Kojima M, Ezura H
  • Organizer: International Symposium on Japanese Solanaceae Genomics Initiative (JSOL) 2013
  • Place of Presentation: 筑波大学
  • Invited
• [Presentation] RNA-seq based Transcript Profiling of Auxin-dependent Fruit Set in Tomato. (2013)
  • Author(s): Shinozaki Y, Hao S, Fei Z, Zhong S, Giovanonni J, Rose JK, Ezura H, Ariizumi T
  • Organizer: International Symposium on Japanese Solanaceae Genomics Initiative (JSOL) 2013
  • Place of Presentation: 筑波大学
  • Invited
• [Presentation] Cytological Dissection and Genetic Interaction of Two Tomato Carpel Mutants (2012)
  • Author(s): Hao S, Ariizumi T and Ezura H
  • Organizer: The 5^<th>Japan-China-Korea Graduate Student Forum
  • Place of Presentation: Tsukuba
• [Presentation] Isolation and cytological characterization of tomato bubble fruit mutants (2012)
  • Author(s): Kimura A, Ariizumi T, Okabe Y, Saito T, Asamizu E, Ezura H
  • Organizer: 第122回日本育種学会
  • Place of Presentation: 京都産業大学
• [Presentation] トマト果実分化機構解明に向けた新規遺伝資源開発 (2012)
  • Author(s): 有泉亨, 木村あかね, 羽尾周平, 森一樹, 江面健太郎, 岡部佳弘, 篠崎良仁, 増田順一郎, 鈴木穣,斎藤岳士, 久原哲, 青木考, 江面浩
  • Organizer: 第122回日本育種学会
  • Place of Presentation: 京都産業大学
• [Presentation] Isolation and cytological characterization of tomato bubble fruit mutants. (2012)
  • Author(s): Kimura A, Ariizumi T, Okabe Y, Saito T, Asamizu E, Ezura H
  • Organizer: 第122回日本育種学会
  • Place of Presentation: 京都産業大学
• [Presentation] Cytological dissection of tomato carpel mutants. (2011)
  • Author(s): Hao S, Ariizumi T, Saito T, Ezura H
  • Organizer: Solanaceae and 2nd Cucurbitaceae Genome Joint Conference, SOL&ICuGI2011
  • Place of Presentation: 神戸国際会議場（神戸市）
• [Presentation] Isolation and cytological characterization of tomato bubble fruit mutants. (2011)
  • Author(s): Kimura A, Ariizumi T, Okabe Y, Saito T, Asamizu E, Ezura H
  • Organizer: Solanaceae and 2nd Cucurbitaceae Genome Joint Conference, SOL&ICuGI2011
  • Place of Presentation: 神戸国際会議場（神戸市）
• [Presentation] Systematic development of Micro-Tom resources: Forward and reverse genetics tools for unraveling the molecular mechanism for tomato fruit development. (2011)
  • Author(s): Ariizumi T, Saito T, Okabe Y, Niinuma K, Asamizu E, Matsukura C, Fukuda N, Mizoguchi T, Bres C, Bernillon S, Moing A, Rothan C, Suzuki H, Aoki K, Ezura H
  • Organizer: Solanaceae and 2nd Cucurbitaceae Genome Joint Conference, SOL&ICuGI2011(招待講演)
  • Place of Presentation: 神戸国際会議場（神戸市）
• [Presentation] Cytological dissection of tomato carpel mutants
  • Author(s): Hao S, Ariizumi T Saito S, Ezura H
  • Organizer: The 8^<th>Solanaceae and 2^<nd>Cucurbitaceae Joint Conference (SOL&ICuGI 2011)
• [Remarks]
  • URL: http://www.gene.tsukuba.ac.jp/Plant/MolecularBreeding/index.html
• [Remarks]
  • URL: http://olericulture-tsukuba.org/manaer
• [Remarks]
  • URL: http://olericulture-tsukuba.org/ariizumi_lab