| Project/Area Number |
23780007
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Breeding science
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| Research Institution | Hokkaido Research Organization |
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Principal Investigator |
YAMASHITA Yoko 地方独立行政法人北海道立総合研究機構, 農業研究本部中央農業試験場, 研究員 (30462386)
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| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 育種学 / 遺伝子 / ジーンサイレンシング / 遺伝学 |
|---|
| Research Abstract |
The expression of the candidate Rsdv1 gene, the major Soybean dwarf virus (SbDV) resistance gene derived from the soybean cultivar 'Wilis', was suppressed by virus-induced gene silencing (VIGS). An evaluation method for SbDV resistance was developed, in which SbDV was detected by ELISA instead of symptom observation. This method enabled resistance evaluation of gene-silenced soybean lines whose symptoms of SbDV are not detectable due to the symptoms caused by VIGS. VIGS of the candidate Rsdv1 gene did not significantly change the percentage of SbDV-infected plants, suggesting that VIGS was not suitable for identification of Rsdv1, or that additional neighboring genes were necessary for resistance.
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