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Identification of the major gene for resistance to Soybean dwarf virus

Research Project

Project/Area Number 23780007
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Breeding science
Research InstitutionHokkaido Research Organization

Principal Investigator

YAMASHITA Yoko  地方独立行政法人北海道立総合研究機構, 農業研究本部中央農業試験場, 研究員 (30462386)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords育種学 / 遺伝子 / ジーンサイレンシング / 遺伝学
Research Abstract

The expression of the candidate Rsdv1 gene, the major Soybean dwarf virus (SbDV) resistance gene derived from the soybean cultivar 'Wilis', was suppressed by virus-induced gene silencing (VIGS). An evaluation method for SbDV resistance was developed, in which SbDV was detected by ELISA instead of symptom observation. This method enabled resistance evaluation of gene-silenced soybean lines whose symptoms of SbDV are not detectable due to the symptoms caused by VIGS. VIGS of the candidate Rsdv1 gene did not significantly change the percentage of SbDV-infected plants, suggesting that VIGS was not suitable for identification of Rsdv1, or that additional neighboring genes were necessary for resistance.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (2 results)

All 2013 2011

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (1 results)

  • [Journal Article] Fine mapping of the major <i>Soybean dwarf virus</i> resistance gene <i>Rsdv1</i> of the soybean cultivar ‘Wilis’2013

    • Author(s)
      山下 陽子、竹内 徹、大西 志全、佐々木 純、田澤 暁子
    • Journal Title

      Breeding Science

      Volume: 63 Issue: 4 Pages: 417-422

    • DOI

      10.1270/jsbbs.63.417

    • NAID

      130004146404

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 「WILIS」由来のダイズわい化病高度抵抗性遺伝子Rsdv1のファインマッピング2011

    • Author(s)
      山下陽子、大西志全、竹内徹
    • Organizer
      日本育種学会第120回講演会
    • Place of Presentation
      福井県立大学
    • Year and Date
      2011-09-24
    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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