Studies on the direction of mutations in relation with gene expression level

• Project/Area Number: 23780008
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Breeding science
• Research Institution: Japan Atomic Energy Agency
• Principal Investigator: HASE Yoshihiro 独立行政法人日本原子力研究開発機構, 量子ビーム応用研究部門, 研究主幹 (70354959)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 植物育種 / 突然変異

Research Abstract

This study focused on the direction of mutation in order to improve the efficiency of plant mutation breeding. The expression level of Arabidopsis genes involved in anthocyanin biosynthesis was elevated by sucrose treatment. The frequency of pigment-less mutants induced by ion beam irradiation was higher in sucrose-treated seedlings than in non-treated seedlings. This suggests the possibility that the highly-expressed genes are relatively mutable.

Research Products

• [Journal Article] Mutational effects of carbon ions near the range end and development of an efficient mutagenesis technique using ion beams (2013)
  • Author(s): Yoshihiro Hase、Ryouhei Yoshihara、Shigeki Nozawa、Issay Narumi
  • Journal Title: JAEA-Review Volume: 2012-046 Pages: 98-98
  • URL: http://www.taka.jaea.go.jp/tiara/j661/annual.html
• [Journal Article] Mutational effects of carbon ions near the range end and development of an efficient mutagenesis technique using ion beams (2013)
  • Author(s): Yoshihiro Hase
  • Journal Title: JAEA-Review Volume: 2012-046 Pages: 98-98
• [Presentation] 飛程末端に近い炭素イオンの変異誘発効果と効率的な変異誘発技術の開発 (2012)
  • Author(s): 長谷純宏
  • Organizer: 第6回高崎量子応用研究シンポジウム
  • Place of Presentation: 高崎シティーギャラリー（高崎市）
• [Presentation] 飛程末端に近い炭素イオンの変異誘発効果と効率的な変異誘発技術の開発
  • Author(s): 長谷純宏、吉原亮平、野澤樹、鳴海一成
  • Organizer: 第6回高崎量子応用研究シンポジウム