Study of relationship between antioxidant activity of soybean flavonoids and chilling stress, and their related gene

• Project/Area Number: 23780009
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Breeding science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: TODA Kyoko 独立行政法人農業・食品産業技術総合研究機構, 作物研究所畑作物研究領域, 主任研究員 (10360447)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ダイズ / 低温ストレス耐性 / フラボノイド / 大豆 / 形質転換 / 高速液体クロマトグラフィー（HPLC）

Research Abstract

Our studies and others have suggested that soybean F3`H gene corresponding to the T locus, which controls pubescence color, is related to chilling stress in soybean. The aim of this study was to clarify the relationship between F3`H and chilling stress using transformed soybean, and its mechanism by morphological study and analyzing flavonoids. A transformant was obtained and gene expression was confirmed by RT-PCR and HPLC analyses of flavonoids. Morphological study and scavenging activity of the DPPH radical indicated that F3`H is localized to the tonoplast in the hilum and funiculus of the immature seed coat, affecting biosynthesis of flavonoid and antioxidant properties in those tissues.

Research Products

• [Journal Article] Linkage mapping, molecular cloning and functional analysis of soybean gene Fg2 encoding flavonol 3-O-glucoside (1 →6) rhamnosyltransferase (2013)
  • Author(s): Rodas FR, Rodriguez TO, Murai Y, Iwashina T, Sugawara S, Suzuki M, Nakabayashi R, Yonekura-Sakakibara K, Saito K, Kitajima J, Toda K, Takahashi R
  • Journal Title: Plant Molecular Biology Volume: 84 Issue: 3 Pages: 287-300
  • DOI: 10.1007/s11103-013-0133-1
  • Peer Reviewed
• [Journal Article] The soybean F3'H protein is localized to the tonoplast in the seed coat hilum (2012)
  • Author(s): Toda, K., et al
  • Journal Title: Planta Volume: 235(In press) Issue: 1 Pages: 79-89
  • DOI: 10.1007/s00425-012-1590-5
  • Peer Reviewed
• [Presentation] Localization and possible role in chilling tolerance of flavonoid 3'-hydroxylase in soybean (2014)
  • Author(s): 戸田恭子、黒岩晴子、Kalaiselvi Senthil、高橋良二
  • Organizer: 第27回国際ポリフェノール会議2014名古屋
  • Place of Presentation: 名古屋大学
• [Presentation] Localization and physiological function of flavonoid 3’-hydroxylase in soybean (2014)
  • Author(s): Kyoko Toda, Haruko Kuroiwa, Kalaiselvi Senthil, Ryoji Takahashi
  • Organizer: 第27回国際ポリフェノール会議 2014 名古屋
  • Place of Presentation: 名古屋大学