Genetic regulation of bolting time in lettuce

• Project/Area Number: 23780023
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Horticulture/Landscape architecture
• Research Institution: The University of Tokyo
• Principal Investigator: LEE ONEW 東京大学, 農学生命科学研究科, 助教 (10447360)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: レタス / 抽だい / QTL解析 / RAD シーケンス / 連鎖地図 / RADマーカー / 抽台

Research Abstract

Lettuce (Lactuca sativa) plants elongate their flower stalks (bolting) when grown under high temperature and long day-length. Here we conducted QTL analysis to know the genetic control of bolting time in lettuce. We used a F5 population developed from two lettuce cultivars Chimasanchu (early bolting type) and Redfire (late bolting type) to measure days to bolting, flowering and seed formation. We developed SNPs markers using RAD-tag sequence and constructed a linkage map. Composite interval mapping detected 2 QTLs for bolting on linkage group 1 and 16. The presence of Redfire in the detected QTL on linkage group 16 accounted for 58% of phenotypic variation. Further study is needed to increase number of markers on linkage map to perform more precise QTL analysis.