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Molecular dissection of species-specific transgene silencing

Research Project

Project/Area Number 23780027
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Horticulture/Landscape architecture
Research InstitutionOsaka Prefecture University

Principal Investigator

MISHIBA Kei-ichiro  大阪府立大学, 生命環境科学研究科(系), 准教授 (70390888)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsDNAメチル化 / リンドウ / 分子育種 / レタス / サイレンシング / 遺伝子組換え / 園芸
Research Abstract

Molecular mechanism of the 35S promoter-specific DNA methylation in gentian was investigated in this study. Two distinct de novo methylated regions were identified after the methylation analysis of an entire T-DNA segment in transgenic gentian. Both of the regions contain two consensus sequences, to which gentian nuclear extracts could bind, and yeast one-hybrid screening identified candidates for binding to the sequences. Additionally, relationship between DNA methylation and histone modification was investigated using transgenic gentian cell suspension culture, and the results showed that de novo methylation of the 35S region occurred in connection with histone modification. To investigate whether the 35S promoter-specific DNA methylation exists in other plant species, transgenic lettuce plants having 35S promoter were produced. As the result, suppression of the transgene expression and hypermethylation of the 35S promoter region were found in the transgenic lettuce plants.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (11 results)

All 2013 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (4 results) Book (1 results) Remarks (3 results)

  • [Journal Article] An attempt to detect siRNA-mediated genomic DNA modification by artificially induced mismatch siRNA in Arabidopsis.2013

    • Author(s)
      Yosuke Miyagawa, Jun Ogawa, Yuji Iwata, Nozomu Koizumi, Kei-ichiro Mishiba
    • Journal Title

      PLOS ONE

      Volume: 8 Issue: 11 Pages: e81326-e81326

    • DOI

      10.1371/journal.pone.0081326

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] De novo DNA methylation of the 35S enhancer revealed by high-resolution methylation analysis of an entire T-DNA segment in transgenic gentian2011

    • Author(s)
      Satoshi Yamasaki, Masayuki Oda, Nozomu Koizumi, Kazuhiko Mitsukuri, Masafumi Johkan, Takashi Nakatsuka, Masahiro Nishihara, Kei-ichiro Mishiba
    • Journal Title

      Plant Biotechnology

      Volume: 28 Issue: 2 Pages: 223-230

    • DOI

      10.5511/plantbiotechnology.10.1222a

    • NAID

      10028259282

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Epigenetic modifications of the 35S promoter in cultured gentian cells2011

    • Author(s)
      Satoshi Yamasaki, Masayuki Oda, Hiroyuki Daimon, Kazuhiko Mitsukuri, Masahumi Johkan, Takashi Nakatsuka, Masahiro Nishihara, Kei-ichiro Mishiba
    • Journal Title

      Plant Science

      Volume: 180(4) Issue: 4 Pages: 612-619

    • DOI

      10.1016/j.plantsci.2011.01.008

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Genetic engineering of eggplant with high carotenoid contents in fruits2013

    • Author(s)
      Tomoya Fujiwara, Shunji Teranishi, Yuki Hiramatsu, Kei-ichiro Mishiba, Nozomu Koizumi
    • Organizer
      JSOL 10th International Symposium on Solanaceae Genomics、Japanese Solanaceae Genomics Initiative (JSOL)
    • Place of Presentation
      大阪府立大学
    • Year and Date
      2013-11-29
    • Related Report
      2013 Final Research Report
  • [Presentation] ナス果実で強く発現する遺伝子のプロモーター解析2012

    • Author(s)
      藤原知也、平松由季、三柴啓一郎、小泉望
    • Organizer
      第30回日本植物細胞分子生物学会大会、日本植物細胞分子生物学会
    • Place of Presentation
      奈良先端科学技術大学院大学
    • Year and Date
      2012-08-05
    • Related Report
      2013 Final Research Report
  • [Presentation] Making transgenic eggplant with high carotenoids contents.、SOL2011 8th Solanaceae and 2nd Cucurbitaceae Genome Joint Conference2011

    • Author(s)
      Tomoya Fujiwara, Shunji Teranishi, Yuki Hiramatsu, Kei-ichiro Mishiba, Nozomu Koizumi
    • Organizer
      Japanese Solanaceae Genomics Initiative (JSOL)
    • Place of Presentation
      神戸コンベンションセンター
    • Year and Date
      2011-11-28
    • Related Report
      2013 Final Research Report
  • [Presentation] リンドウに導入したT-DNA領域全長のde novo DNAメチル化解析2011

    • Author(s)
      山崎識知ら
    • Organizer
      日本育種学会
    • Place of Presentation
      横浜市立大学(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Book] The Gentianaceae, Vol.2. Biotechnology and Application, Chapter 10. Genetic transformation and its application to gentian breeding2013

    • Author(s)
      Jan J. Rybczynski, Michael R. Davey, Anna Mikula (eds.) M. Nishihara, K. Mishiba, T. Imamura, H. Takahashi, T. Nakatsuka
    • Total Pages
      250
    • Publisher
      Springer
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.plant.osakafu-u.ac.jp/~mishiba/

    • Related Report
      2013 Final Research Report
  • [Remarks] 申請者の研究室HP(研究紹介)

    • URL

      http://www.plant.osakafu-u.ac.jp/~mishiba/Research.html

    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://www.plant.osakafu-u.ac.jp/~mishiba/Research.html

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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