| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Outline of Final Research Achievements |
Flavonoids have received much attention for their potential contribution to human health. We performed the detailed analysis of the composition of each flavonoid as well as comprehensive gene expression analysis in the grape berries cultured in various environment. Genes which were coordinately expressed with proanthocyanidin (PA) accumulation under different light conditions, and a new PA regulator, VvMYBPAR, was identified. PA-specific regulation of VvMYBPAR was confirmed by VvMYBPAR constitutive expression in Arabidopsis and Grapevine as well as transient reporter assay using grapevine cells. VvMYBPAR showed a unique spatiotemporal expression during berry development as well as responce to environmental stimuli, in contrast to already identified PA regulators, suggesting the regulation of different PA-specific branch genes. This new factor suggested the polygenic regulation of PA biosynthesis in grapes by closely related MYB transcription factors.
|
