| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Research Abstract |
RNA silencing is a sequence-specific RNA degradation mechanism conserved among eukaryotes, and act as a defence mechanism against fungal virus (mycovirus) in filamentous fungi. In this study, we analyzed induction and suppression of RNA silencing in white root rot fungus, Rosellinia necatrix by mycovirus infection. We revealed that a mycoreovirus (Rosellinia necatrix mycoreovirus 3; RnMyRV3) suppresses RNA silencing in R. necatrix. On the other hand, by deep-sequencing analysis of small RNA which is a hallmark of induction of RNA silencing, we showed that mycovirus-derived small RNA accumulates in R. necatrix strains infected with each of five mycoviruses including RnMyRV3 and other four unrelated mycoviruses, indicating that mycoviruses induce RNA silencing in R. necatrix.
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