Analysis of induction and suppression of RNA silencing by mycovirus infection
| Project/Area Number |
23780045
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant pathology
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
YAEGASHI Hajime 独立行政法人農業・食品産業技術総合研究機構, 果樹研究所リンゴ研究領域, 研究員 (90582594)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | マイコウイルス / サイレンシング / サイレンシングサプレッサー / 白紋羽病菌 / レオウイルス / スモールRNA / 次世代シーケンス / 菌類ウイルス / RNAサイレンシング / サプレッサー / siRNA / 高速シーケンス / マイコレオウイルス3 |
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| Research Abstract |
RNA silencing is a sequence-specific RNA degradation mechanism conserved among eukaryotes, and act as a defence mechanism against fungal virus (mycovirus) in filamentous fungi. In this study, we analyzed induction and suppression of RNA silencing in white root rot fungus, Rosellinia necatrix by mycovirus infection. We revealed that a mycoreovirus (Rosellinia necatrix mycoreovirus 3; RnMyRV3) suppresses RNA silencing in R. necatrix. On the other hand, by deep-sequencing analysis of small RNA which is a hallmark of induction of RNA silencing, we showed that mycovirus-derived small RNA accumulates in R. necatrix strains infected with each of five mycoviruses including RnMyRV3 and other four unrelated mycoviruses, indicating that mycoviruses induce RNA silencing in R. necatrix.
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Report
(4 results)
Research Products
(17 results)
