Functional analysis of a novel phosphorylation regulator in MAMPs signaling
Project/Area Number |
23780048
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Plant pathology
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Research Institution | The Institute of Physical and Chemical Research |
Principal Investigator |
MATSUI Hidenori 独立行政法人理化学研究所, 植物プロテオミクス研究ユニット, 特別研究員 (20598833)
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Project Period (FY) |
2011 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 植物免疫 / プロテオミクス / リン酸化 / シグナル伝達 / シロイヌナズナ / リン酸化プロテオミクス |
Research Abstract |
To identify the phosphorylation signal network in plant immunity, we performed screening of T-DNA insertion lines in Arabidopsis to determine proteins with changed phosphorylation status in response to MAMP treatment as indicated by measurement of reactive oxygen species (ROS) by flg22 treatment. We identified 38 genes that showed abnormal ROS production after flg22 treatment. Additionally, some candidates exhibited the abnormal immune responses against bacterial pathogen infection, suggesting that phosphoproteomic approaches have the potential to identify novel immune signaling components in plants.
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Report
(3 results)
Research Products
(39 results)
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[Presentation] 植物免疫シグナル解明の為のリン酸化プロテオミクス手法の確立2012
Author(s)
Hidenori Matsui, Yuko Nomura, Fumiko Kato, Ken Shirasu, Hirofumi Nakagami
Organizer
Establishment of phosphoproteomic approaches for plant signaling dissection
Place of Presentation
Nara, Japan
Related Report
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