| Project/Area Number |
23780051
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied entomology
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| Research Institution | Tokushima Bunri University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 昆虫病理 / IAPV / 殻タンパク質 / RNA合成酵素 / ミツバチ / 組換えタンパク質 |
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| Research Abstract |
Colony Collapse Disorder (CCD) is a serious problem threatening the health of honeybees and the economic stability of commercial beekeeping and pollination operations in the world. In this study, we focused on Israeli Acute Paralysis Virus (IAPV), which is considered as a candidate reason for CCD, and challenged to investigate the structure and function of the capsid protein and the RNA polymerase in IAPV. Recombinant proteins of the capsid proteins were expressed, solubilized and purified by combinational expression of 2 or 3 kinds of subunit proteins. Next, we found 2 proteins, which seemed to interact with these recombinant capsid protein subunits, from honeybee brains and guts. Two of 6 partial recombinant proteins of RNA polymerase was successful for expression and solubilization. We will try to isolate the proteins in honeybee tissues which interact with these recombinant proteins.
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