| Project/Area Number |
23780055
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied entomology
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
CORNETTE Richard 独立行政法人農業生物資源研究所, 昆虫機能研究開発ユニット, 主任研究員 (20376586)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ネムリユスリカ / 乾燥耐性 / 酸化ストレス / マイクロアレイ解析 / RNAi / 培養細胞 / Cnc-C / 細胞周期 / 細胞分裂 / Keap1 |
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| Research Abstract |
Larvae of the sleeping chironomid (Polypedilum vanderplanki) can survive total desiccation in an ametabolic state known as anhydrobiosis. Gene expression during the recovery process was investigated by microarray. Whereas stress genes, such as antioxidants, were still expressed during the resuming of metabolism just after rehydration, 24 h later, we observed genes involved in the repair of biological molecules. DNA repair is tightly associated with cell cycle but synchronization of cell cycle was not detected during anhydrobiosis. On the other hand, oxidative stress induced anhydrobiosis-related gene expression. Consequently, reactive oxygen species could be the main trigger of anhydrobiosis. Thus, we characterized the transcription factor involved in oxidative stress sensing, Cnc-C, whose expression was up-regulated upon desiccation. RNAi experiments on P. vanderplanki cell cultures suggested that Cnc-C was involved in the induction of anhydrobiosis-related gene expression.
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