Understanding the sex-determining processes and the molecular mechanism of reproductive manipulations in insects
| Project/Area Number |
23780056
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied entomology
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
KAGEYAMA Daisuke 独立行政法人農業生物資源研究所, 昆虫微生物機能研究ユニット, 主任研究員 (60401212)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 性転換 / 移植 / Wolbachia / 昆虫 / 共生微生物 / カイコ / dsx / 共生細菌 / 生殖操作 / ショウジョウバエ / 性決定 |
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| Research Abstract |
When transinfected into a male Bombyx mori cell line (M1), feminizing Wolbachia endosymbiont derived from Eurema mandarina affected sex-specific splicing of the sex-determining gene doublesex (dsx). Two to four weeks after transinfection, dsx begins to exhibit splicing products of both male and female. Preliminary results of the macroarray analyses demonstrated dozens of candidate genes affected by the feminizing Wolbachia, which are currently under further analyses. The feminizing Wolbachia was successfully transinfected into a male Drosophila cell line (S2), but the splicing pattern of dsx remained unchanged.
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Report
(3 results)
Research Products
(19 results)
