Structural analysis of plant borate transporter
| Project/Area Number |
23780060
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KASAI Koji 東京大学, 大学院・農学生命科学研究科, 特任准教授 (80517938)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 植物栄養代謝 / ホウ酸トランスポーターBOR / 結晶構造解析 |
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| Research Abstract |
We screened conditions for solubilization, purification, and crystallization of plant BOR borate transporter. We found that combination of Arabidopsis BOR4 and detergent dodecylmaltoside (DDM) showed high monodispersity. Furthermore, addition of DIDS (anion exchanger inhibitor) or borate increased thermal stability of BOR4-DDM complex.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] VvBOR1, the Grapevine Ortholog of AtBOR1, Encodes and Efflux Boron Transporter That is Differencially Expressed Throughout Reproductive Development of Vitis vinifera L2012
Author(s)
Perez-Castro, R., Kasai, K., Gainza-Cortes, F., Ruiz-Lara, S., Casaretto, J., Pena-Cortes, H., Tapia, J. and Fujiwara, T. Gonzalez E.
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Journal Title
Plant Cell Physiol
Volume: 53
Issue: 2
Pages: 485-494
DOI
Related Report
Peer Reviewed
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