Structural analysis of plant borate transporter

• Project/Area Number: 23780060
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant nutrition/Soil science
• Research Institution: The University of Tokyo
• Principal Investigator: KASAI Koji 東京大学, 大学院・農学生命科学研究科, 特任准教授 (80517938)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 植物栄養代謝 / ホウ酸トランスポーターBOR / 結晶構造解析

Research Abstract

We screened conditions for solubilization, purification, and crystallization of plant BOR borate transporter. We found that combination of Arabidopsis BOR4 and detergent dodecylmaltoside (DDM) showed high monodispersity. Furthermore, addition of DIDS (anion exchanger inhibitor) or borate increased thermal stability of BOR4-DDM complex.

Research Products

• [Journal Article] Polar localization and endocytic degradation of a boron transporter BOR1 dependent on specific tyrosine residues (2012)
  • Author(s): Yoshinari, A., Kasai, K., Fujiwara, T., Naito, S. and Takano, J.
  • Journal Title: Plant Signal Behav Volume: 7 Issue: 1 Pages: 46-9
  • DOI: 10.4161/psb.7.1.18527
  • Peer Reviewed
• [Journal Article] VvBOR1, the Grapevine Ortholog of AtBOR1, Encodes and Efflux Boron Transporter That is Differencially Expressed Throughout Reproductive Development of Vitis vinifera L (2012)
  • Author(s): Perez-Castro, R., Kasai, K., Gainza-Cortes, F., Ruiz-Lara, S., Casaretto, J., Pena-Cortes, H., Tapia, J. and Fujiwara, T. Gonzalez E.
  • Journal Title: Plant Cell Physiol Volume: 53 Issue: 2 Pages: 485-494
  • DOI: 10.1093/pcp/pcs001
  • Peer Reviewed
• [Journal Article] Low-affinity cation transporter (OsLCT1) regulates cadmium transport into rice grains (2011)
  • Author(s): Shimpei URAGUCHI
  • Journal Title: Proceedings of the National Academy of Sciences, USA Volume: 108 Issue: 52 Pages: 20959-20964
  • DOI: 10.1073/pnas.1116531109
  • Peer Reviewed
• [Presentation] ホウ酸トランスポーターBOR1の極性形成およびホウ素濃度に応答した分解制御の生理学的意義 (2013)
  • Author(s): 笠井光治、藤原徹
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学
• [Presentation] ホウ酸トランスポーターBOR1の極性形成およびホウ素濃度に応答した分解制御の生理的意義 (2013)
  • Author(s): 笠井光治
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学
• [Presentation] Mutational Analysis of Arabidopsis BOR1 Borate Transporter to Identify the Amino Acid Residues Involved in Polar-Localization (2013)
  • Author(s): Koji Kasai
  • Organizer: 17th International Plant Nutrition Colloquium (IPNC2013)
  • Place of Presentation: Istanbul Convention and Exhibition Center (ICEC)
• [Presentation] The Role of Boron-Induced Degradation of Arabidopsis BOR1 Borate Transporter for Boron Homeostasis (2013)
  • Author(s): Koji Kasai
  • Organizer: Boron Satellite Meeting of IPNC2013
  • Place of Presentation: Istanbul Convention and Exhibition Center (ICEC)
• [Presentation] ホウ酸トランスポーターBOR1の極性欠損変異体の解析 (2012)
  • Author(s): 笠井光治、高野順平、藤原徹
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
• [Presentation] ホウ酸トランスポーターBOR1の極性欠損変異体の解析 (2012)
  • Author(s): 笠井光治、高野順平、藤原徹
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学（京都府）
• [Presentation] シロイヌナズナホウ酸トランスポーターBOR1の細胞内偏在機構の解析 (2011)
  • Author(s): 笠井光治、高野順平、藤原徹
  • Organizer: 日本土壌肥料学会2011年度つくば大会
  • Place of Presentation: つくば国際会議場
• [Presentation] シロイヌナズナホウ酸トランスポーターBOR1の細胞内偏在機構の解析 (2011)
  • Author(s): 笠井光治、高野順平、藤原徹
  • Organizer: 日本土壌肥料学会2011年度つくば大会
  • Place of Presentation: つくば国際会議場（茨城県）
• [Remarks]
  • URL: http://park.itc.u-tokyo.ac.jp/syokuei/