Analyses of cell-type specific MATE transporters in the nodules

• Project/Area Number: 23780066
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant nutrition/Soil science
• Research Institution: Kyoto University
• Principal Investigator: SUGIYAMA Akifumi 京都大学, 生存圏研究所, 助教 (20598601)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 共生窒素固定 / 根粒 / ミヤコグサ / MATE型輸送体 / クエン酸 / フラボノイド

Research Abstract

Cell-type specific RNA was extracted with Lasermicrodissection and used for microarray analysis. In this study, expression and functional analyses of cell-type specific MATE transporters in the nodules were performed. LjMATE1 was expressed in the infection zone of nodules and found to transport citrate for Fe translocation to the infection zone of nodules. LjMATE2 was expressed highly in the anthocyanin-accumulating stems and found to be expressed at the tonoplasts. The expression of LjMATE3 was induced during nodulation and also by GSH in a similar way as genes involved in the flavonoid synthesis.

Research Products

• [Journal Article] EjMATEI, a citrate transporter responsible for iron supply to nodule infection zone of Lotus japonicus. (2013)
  • Author(s): Takanashi, K., et al.
  • Journal Title: Plant Cell Physiol. Volume: 54 Issue: 4 Pages: 585-594
  • DOI: 10.1093/pcp/pct019
  • Peer Reviewed
• [Journal Article] Tissue-specific transcriptome analysis in nodules of Lotus japonicus (2012)
  • Author(s): Takanashi, K., et al
  • Journal Title: Mol Plant Microbe Interact. Volume: 25(in press) Issue: 7 Pages: 869-876
  • DOI: 10.1094/mpmi-01-12-0011-r
  • Peer Reviewed
• [Journal Article] Tissue-specific transcriptome analysis in nodules of Lotus japonicus. (2012)
  • Author(s): Takanashi, K,, Takahashi, H., Sakurai, N., Sugiyama, A., Suzuki, H., Shibata, D., Nakazono, M., Yazaki, K.
  • Journal Title: Mol. Plant Microbe Interact. Volume: in press
  • Peer Reviewed
• [Presentation] Analysis of MATE-type transporters, LjMATE2 and LjMATE3 in Lotus japonicus (2013)
  • Author(s): Yoshihiro Ota, Kojiro Takanashi, Akifumi Sugiyama, Toshio Aoki, Kazufumi Yazaki
  • Organizer: International Workshop on Plant Membrane Biology XVI
  • Place of Presentation: 倉敷市芸文館、倉敷市
  • Year and Date: 2013-03-28
• [Presentation] ミヤコグサのMATE型輸送体LjMATE2およびLjMATE3の解析 (2013)
  • Author(s): 太田喜寛, 高梨功次郎, 杉山暁史, 青木俊夫,矢崎一史
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学、岡山市
  • Year and Date: 2013-03-22
• [Presentation] ミヤコグサのMATE型輸送体 LjMATE2およびLjMATE3の解析 (2013)
  • Author(s): 太田 喜寛
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学（岡山市）
• [Presentation] Analysis of MATE-type transporters, LjMATE2 and LjMATE3 in Lotus japonicus (2013)
  • Author(s): 杉山 暁史
  • Organizer: International Workshop on Plant Membrane Biology XVI
  • Place of Presentation: 倉敷市芸文館（倉敷市）
• [Presentation] ミヤコグサで発現するMATE型輸送体LjMATE2 および LjMATE3の解析 (2012)
  • Author(s): 太田喜寛, 高梨功次郎, 杉山暁史, 青木俊夫,矢崎一史
  • Organizer: 第22回植物微生物研究交流会
  • Place of Presentation: 神戸大学、神戸市
  • Year and Date: 2012-09-26
• [Presentation] ミヤコグサで発現するMATE型輸送体LjMATE2 および LjMATE3の解析 (2012)
  • Author(s): 太田 喜寛
  • Organizer: 第22回植物微生物研究会交流会
  • Place of Presentation: 神戸大学（神戸市）
• [Presentation] ダイズ根においてフラボノイド配糖体を分泌する輸送体の探索 (2011)
  • Author(s): 山下 和晃、杉山 暁史、矢崎 一史
  • Organizer: 第21回植物微生物研究交流会
  • Place of Presentation: 岡山大学、岡山市
  • Year and Date: 2011-09-21
• [Presentation] ミヤコグサ根粒におけるMATE型輸送体LjMATE2 および LjMATE3の発現解析 (2011)
  • Author(s): 太田喜寛, 高梨功次郎, 杉山暁史, 矢崎一史
  • Organizer: 第21回植物微生物研究交流会
  • Place of Presentation: 岡山大学、岡山市
  • Year and Date: 2011-09-21
• [Presentation] ミヤコグサ根粒におけるMATE型輸送体LjMATE2 および LjMATE3の発現解析 (2011)
  • Author(s): 太田喜寛, 高梨功次郎, 杉山暁史, 矢崎一史
  • Organizer: 第21回植物微生物研究交流会
  • Place of Presentation: 岡山大学
• [Presentation] ダイズ根においてフラボノイド配糖体を分泌する輸送体の探索 (2011)
  • Author(s): 山下 和晃、杉山 暁史、矢崎 一史
  • Organizer: 第21回植物微生物研究交流会
  • Place of Presentation: 岡山大学
• [Book] Secretions and Exudates in Biological Systems (2012)
  • Author(s): Sugiyama, A. and Yazaki, K.
  • Total Pages: 32
  • Publisher: Springer
• [Remarks]
  • URL: http://www.rish.kyoto-u.ac.jp/W/LPGE/
• [Remarks]
  • URL: http://www.rish.kyoto-u.ac.jp/W/LPGE/index.html