| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Research Abstract |
Silicon transporter Lsi1 is involved in high accumulation of Si in rice. Lsi1 is specifically localized at both the exodermis and endodermis in the root and the mRNA expression levels of these transporter genes are down-regulated in response to the accumulation of Si in the shoot. To understand the mechanism regulating the expression and localization of Lsi1, I performed promoter deletion assay. As a result, the candidate region of ciselement for cell-specificity of the Lsi1 localization was located at -1600bp to -1420bp in the upstream. Furthermore, similar analysis with transgenic plants showed that candidate region for the down-regulation of mRNA was located at -600 to -400bp in the upstream of Lsi1.
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