Development of low-carbon bioelectrode systems to catalyze fuel gas production

• Project/Area Number: 23780074
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: The University of Tokyo
• Principal Investigator: KOBAYASHI Hajime 東京大学, 工学(系)研究科(研究院), 准教授 (50549269)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: バイオエネルギー / エネルギー変換 / バイオ水素 / バイオメタン / 生電気化学的反応 / バイオカソード / 生電気化学的活性 / 電子放出細菌 / バイオガス / メタン / 水素 / メタン菌

Research Abstract

Biocathodes capable of catalyzing CH4 and H2 production in bio-electrochemical systems were established by using thermophilic microorganisms as biocatalysts. The CH4-producing biocathode showed the highest electromethanogenic activity so far reported with the current to CH4 conversion efficiency of over 95%. The electrochemical analyses showed that the biocathodic electromethanogenesis was catalyzed via two pathways depend on the cathode potential. The H2-producing biocathode showed the current to H2 conversion efficiency of ca. 80%, which was comparable to those of the metal catalysts. Molecular phylogenetic analyses of microbial consortia associated with the biocathodes suggested a possibility that diverse thermophiles have catalytic activities for electrochemical reactions.

Research Products

• [Journal Article] CO_2から資源を生み出す微生物電解合成システム (2014)
  • Author(s): 小林肇,佐藤光三
  • Journal Title: 水素エネルギー協会(HESS)会誌 Volume: 39 Pages: 24-32
• [Journal Article] CO2から資源を生み出す微生物電解合成システム (2014)
  • Author(s): 小林 肇, 佐藤光三
  • Journal Title: 水素エネルギー協会(HESS)会誌 Volume: 39 Pages: 24-32
• [Journal Article] Bioelectrochemical analyses of a thermophilic biocathode catalyzing sustainable hydrogen production. (2013)
  • Author(s): Q. Fu, H. Kobayashi, Y. Kuramochi, J. Xu, T. Wakayama, H. Maeda and K. Sato
  • Journal Title: Int J Hydrogen Energ Volume: 38 Issue: 35 Pages: 15638-45
  • DOI: 10.1016/j.ijhydene.2013.04.116
  • Peer Reviewed
• [Journal Article] A thermophilic Gram-negative nitrate-reducing bacterium, Calditerrivibrio nitroreducens, exhibiting electricity generation capability (2013)
  • Author(s): Q. Fu, H. Kobayashi, H. Kawaguchi, T. Wakayama, H. Maeda and K. Sato
  • Journal Title: Environ Sci Technol Volume: 47 Issue: 21 Pages: 12583-90
  • DOI: 10.1021/es402749f
  • Peer Reviewed
• [Journal Article] Mechanism of electromethanogenic reduction of CO_2 by a thermophilic methanogen (2013)
  • Author(s): M. Hara, Y. Onaka, H. Kobayashi, Q. Fu, H. Kawaguchi, J. Vilcaez and K. Sato
  • Journal Title: Energy Procedia Volume: 37 Pages: 7021-8
  • Peer Reviewed
• [Journal Article] Identification of new microbial mediators for electromethanogenic reduction of geologically-stored carbon dioxide (2013)
  • Author(s): Q. Fu, H. Kobayashi, H. Kawaguchi, J. Vilcaez and K. Sato
  • Journal Title: Energy Procedia Volume: 37 Pages: 7006-13
  • Peer Reviewed
• [Journal Article] Electromethanogenic CO_2 conversion by subsurface-reservoir microorganisms (2013)
  • Author(s): Y. Kuramochi, Q. Fu, H. Kobayashi, M. Ikarashi, T. Wakayama, H. Kawaguchi, J. Vilcaez, H. Maeda and K. Sato
  • Journal Title: Energy Procedia Volume: 37 Pages: 7014-20
  • Peer Reviewed
• [Journal Article] Electrochemical and microbial analysis of the bio-electrodes of the electromethanogenic reactor. (2013)
  • Author(s): H. Kobayashi, N. Saito, Q. Fu, H. Kawaguchi, J. Vilcaez, T. Wakayama, H. Maeda and K. Sato
  • Journal Title: J Biosci Bioeng Volume: 115 Issue: 1 Pages: 114-7
  • DOI: 10.1016/j.jbiosc.2013.01.001
  • Peer Reviewed
• [Journal Article] Bioelectrochemical analyses of a thermophilic biocathode catalyzing sustainable hydrogen production. (2013)
  • Author(s): Q. Fu, H. Kobayashi, Y. Kuramochi, J. Xu, T. Wakayama, H. Maeda and K. Sato.
  • Journal Title: International Journal of Hydrogen Energy Volume: 38
  • Peer Reviewed
• [Journal Article] Electrochemical and phylogenetic analyses of current-generating microorganisms in a thermophilic microbial fuel cell. (2012)
  • Author(s): Q. Fu, H. Kobayashi, H. Kawaguchi, J. Vilcaez, T. Wakayama, H. Maeda and K. Sato
  • Journal Title: J Biosci Bioeng Volume: 115 Issue: 3 Pages: 268-71
  • DOI: 10.1016/j.jbiosc.2012.10.007
  • NAID: 110009596099
  • Peer Reviewed
• [Presentation] Thermophilic bio-electrochemical systems for CO2-to-methane conversion technology (invited lecture) (2014)
  • Author(s): H. Kobayashi
  • Organizer: The 1st ASEAN Microbial Biotechnology Conference 2014 (AMBC2014)
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2014-02-20
  • Invited
• [Presentation] Electromethanogenesis : CO2からカーボンニュートラルな資源を生み出す (2013)
  • Author(s): 小林肇
  • Organizer: 第21回静岡フォーラム/第46回研究交流セミナー・メタン利用技術の最前線
  • Place of Presentation: 静岡
  • Year and Date: 2013-12-11
  • Invited
• [Presentation] Electromethanogenesis : CO2からカーボンニュートラルな資源を生み出す (2013)
  • Author(s): 小林肇
  • Organizer: 第五回システム創成学学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2013-12-07
• [Presentation] Electrochemical evidence of direct electron transfer in a thermophilic biocathode for carbon dioxide reduction (2013)
  • Author(s): Q. Fu, H. Kobayashi, T. Wakayama, H. Maeda, K. Sato
  • Organizer: BioH2 2013 Conference, Montreal
  • Place of Presentation: Canada
  • Year and Date: 2013-08-07
• [Presentation] 持続型炭素循環システム (2013)
  • Author(s): 小林肇
  • Organizer: さいたま・水とみどりのアカデミー
  • Place of Presentation: 埼玉
  • Year and Date: 2013-07-24
  • Invited
• [Presentation] Identification of new microbial mediators for electromethanogenic reduction of geologically-stored carbon dioxide (2012)
  • Author(s): Q. Fu, H. Kobayashi, H. Kawaguchi, J. Vilcáez, K. Sato
  • Organizer: 11th International Conference on Greenhouse Gas Control Technologies (GHGT-11)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2012-11-22
• [Presentation] Exoelectrogenic ability of thermophilic microorganisms from various environments (2012)
  • Author(s): Q. Fu, H. Kobayashi, H. Kawaguchi, J. Vilcaez, K. Sato
  • Organizer: American Chemical Society 243th National Meeting & Exposition
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2012-03-29
• [Presentation] Bioelectrochemical analyses of a thermophilic biocathode catalyzing sustainable hydrogen production (2012)
  • Author(s): Q. Fu, H. Kobayashi, Y. Kuramochi, J. Xu, K. Sato
  • Organizer: The 2012 Asian Biohydrogen and Bioproducts Symposium
  • Place of Presentation: 重慶,中国
• [Presentation] Identification of new microbial mediators for electromethanogenic reduction of geologically-stored carbon dioxide. (2012)
  • Author(s): Q. Fu, H. Kobayashi, H. Kawaguchi, J. Vilcáez, K. Sato.
  • Organizer: 11th International Conference on Greenhouse Gas Control Technologies (GHGT-11)
  • Place of Presentation: 国立京都国際会館、京都
• [Presentation] Mechanism of electromethanogenic reduction of CO2 by a thermophilic methanogen. (2012)
  • Author(s): M. Hara, Y. Onaka, H. Kobayashi, Q. Fu, H. Kawaguchi, J. Vilcáez, K. Sato.
  • Organizer: 11th International Conference on Greenhouse Gas Control Technologies (GHGT-11)
  • Place of Presentation: 国立京都国際会館、京都
• [Presentation] Electromethanogenic CO2 conversion by subsurface-reservoir microorganisms. (2012)
  • Author(s): Y. Kuramochi, Q. Fu, H. Kobayashi, M. Ikarashi, T. Wakayama, H. Kawaguchi, J. Vilcáez, H. Maeda, K. Sato.
  • Organizer: 11th International Conference on Greenhouse Gas Control Technologies (GHGT-11)
  • Place of Presentation: 国立京都国際会館、京都
• [Presentation] Bioelectrochemical analyses of a thermophilic biocathode catalyzing sustainable hydrogen production. (2012)
  • Author(s): Q. Fu, H. Kobayashi, Y. Kuramochi, J. Xu, K. Sato.
  • Organizer: The 2012 Asian Biohydrogen and Bioproducts Symposium
  • Place of Presentation: 重慶, 中国
• [Presentation] Thermophilic bio-electrochemical systems for CO2-to-methane conversion technology
  • Author(s): H. Kobayashi
  • Organizer: The 1st ASEAN Microbial Biotechnology Conference 2014 (AMBC2014)
  • Place of Presentation: Bangkok, Thailand
  • Invited
• [Presentation] Electromethanogenesis: CO2からカーボンニュートラルな資源を生み出す
  • Author(s): 小林 肇
  • Organizer: 第21回静岡フォーラム/第46回研究交流セミナー・メタン利用技術の最前線
  • Place of Presentation: 静岡県、浜松市、アクトシティ浜松
  • Invited
• [Presentation] Electromethanogenesis: CO2からカーボンニュートラルな資源を生み出す
  • Author(s): 小林 肇
  • Organizer: 第五回システム創成学学術講演会
  • Place of Presentation: 東京都、文京区、東京大学
• [Presentation] Microbial fuel cells (MFC) in thermophilic conditions
  • Author(s): R. Umeda, Q. Fu, H. Kobayashi, K. Sato
  • Organizer: 日本微生物生態学会第29回大会
  • Place of Presentation: 鹿児島県、鹿児島市、鹿児島大学
• [Presentation] Electricity generation by a microbial fuel cell operated under hyperthemophilic conditions
  • Author(s): H. Tamamura, Q. Fu, H. Kobayashi, T. Wakayama, H. Maeda, K. Sato
  • Organizer: 日本微生物生態学会第29回大会
  • Place of Presentation: 鹿児島県、鹿児島市、鹿児島大学
• [Presentation] Analysis of electromethanogenesis mechanism of thermophilic biocathode
  • Author(s): J. Xu, Q. Fu, H. Kobayashi, T. Wakayama, H. Maeda, K. Sato
  • Organizer: 日本微生物生態学会第29回大会
  • Place of Presentation: 鹿児島県、鹿児島市、鹿児島大学
• [Presentation] Electrochemical evidence of direct electron transfer in a thermophilic biocathode for carbon dioxide reduction
  • Author(s): Q. Fu, H. Kobayashi, T. Wakayama, H. Maeda, K. Sato
  • Organizer: BioH2 2013 Conference
  • Place of Presentation: Montreal, Canada
• [Presentation] 持続型炭素循環システム.” さいたま・水とみどりのアカデミー, 埼玉
  • Author(s): 小林 肇
  • Organizer: さいたま・水とみどりのアカデミー
  • Place of Presentation: 埼玉県、さいたま市、浦和パルコ
  • Invited
• [Presentation] Exoelectrogenic ability of thermophilic microorganisms from various environments
  • Author(s): 付 乾、小林 肇、川口 秀夫、ハビエル ビルカエス、佐藤 光三
  • Organizer: American Chemical Society 243th National Meeting & Exposition
  • Place of Presentation: San Diego Convention Center, San Diego, California, USA
• [Presentation] メタン生成的条件下における電気化学的メタン生成活性を持つ微生物群の解析
  • Author(s): 齋藤 直輝、付 乾、川口 秀夫、ハビエル ビルカエス、小林 肇、佐藤 光三
  • Organizer: 日本微生物生態学会第27回大会
  • Place of Presentation: 京都大学、京都
• [Presentation] メタン菌による電気化学的メタン生成反応の解析と数値モデル化
  • Author(s): 大中 温、付 乾、川口 秀夫、ハビエル ビルカエス、小林 肇、佐藤 光三
  • Organizer: 日本微生物生態学会第27回大会
  • Place of Presentation: 京都大学、京都
• [Presentation] Analysis of microbial activity in thermophilic bioelectrochemical reactors
  • Author(s): 付 乾、小林 肇、川口 秀夫、ハビエル ビルカエス、佐藤 光三
  • Organizer: 日本微生物生態学会第27回大会
  • Place of Presentation: 京都大学、京都
• [Book] "枯渇油・ガス田をジオバイオリアクターとしたバイオエネルギー生産の可能性."in光合成のエネルギー利用と環境応用 (2014)
  • Author(s): 若山樹,小林肇,前田治男
  • Publisher: シーエムシー出版
• [Book] 枯渇油・ガス田をジオバイオリアクターとしたバイオエネルギー生産の可能性.” in 光合成のエネルギー利用と環境応用 (2013)
  • Author(s): 若山 樹, 小林 肇, 前田治男
  • Total Pages: 12
  • Publisher: シーエムシー出版
• [Remarks]
  • URL: http://escc.frcer.t.u-tokyo.ac.jp
• [Remarks] http://escc.frcer.t.u-tokyo.ac.jp/index.html
• [Remarks]
  • URL: http://escc.frcer.t.u-tokyo.ac.jp/index.html