Construction of in vitro artificial metabolic pathway using thermo-tolerant enzymes
Project/Area Number |
23780078
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Applied microbiology
|
Research Institution | Osaka University |
Principal Investigator |
HONDA Kohsuke 大阪大学, 工学(系)研究科(研究院), 准教授 (90403162)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 酵素反応 / 発酵 / 生体機能利用 / 代謝工学 / 好熱菌 / ピルビン酸酸化 / 合成代謝工学 / タンパク質工学 / ピルビン酸デヒドロゲナーゼ / 好熱性菌 / 耐熱性酵素 |
Research Abstract |
An artificial pyruvate decarboxylation pathway, through which pyruvate can be converted to acetyl-CoA, was constructed by using a coupled enzyme system consisting of thermo-tolerant pyruvate decarboxylase and CoA-acylating aldehyde dehydrogenase. To demonstrate the applicability of the artificial pathway for chemical production, a cofactor-balanced and CoA-recycling synthetic pathway for N-acetylglutamate production was designed by coupling the acetyl-CoA-forming pathway with the glutamate dehydrogenase and N-acetylglutamate synthetase. N-Acetylglutamate could be produced from an equimolar mixture of pyruvate and alpha-ketoglutarate with a molar yield of 55% through the synthetic pathway consisting of the four thermo-tolerant enzymes. The overall recycling number of CoA was calculated to be 27.
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Report
(4 results)
Research Products
(22 results)