Development of genome-engineering technology and its application to genome evolution in Saccharomyces cerevisiae
| Project/Area Number |
23780080
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Research Collaborator |
HARASHIMA Satoshi 大阪大学, 大学院・工学研究科, 教授 (70116086)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 部分異数性 / ゲノム / 育種 / 出芽酵母 / 染色体分断 / エボリューション / 循環型社会 |
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| Research Abstract |
Using PCR-mediated chromosomal duplication and deletion technologies that we developed, we investigated whether segmental duplication or deletion of specific chromosomal regions improved industrially important phenotypes in Saccharomyces cerevisiae. Systematic analysis of segmental duplication and deletion of chromosomes revealed that alteration of copy number of specific chromosomal regions enhanced resistance against a variety of stresses including ethanol, high-temperature and acid stresses. These results indicate that the genome-engineering technologies provide an unique and useful tool not only for analyzing genome function but also for breeding of S. cerevisaie for efficient production of bioethanol or organic acids.
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Report
(3 results)
Research Products
(23 results)
