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Infection of filamentous phages causes several physiological changes to the phytopathogen bacterium.

Research Project

Project/Area Number 23780082
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied microbiology
Research InstitutionHiroshima University

Principal Investigator

KAWASAKI Takeru  広島大学, 先端物質科学研究科, 助教 (00510299)

Project Period (FY) 2011-04-28 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords分子生物学 / 植物病原菌 / 青枯病菌 / ファージ
Research Abstract

The phytopathogenic gram-negative bacterium Ralstonia solanacearum causes lethal bacterial wilt more than 200species in 50 botanical families, including economically important crops. Although some management strategies are tried to control this disease such as chemicals control, crop rotation and resistant plants, they are unfriendly to the environment and economics.We reported that infection of filamentous type phages (RSS type and RSM type) causes several physiological changes to host cells, twitching motility, amount of extracellular polysaccharide and especially virulence.
In the case of RSS infection: increase of virulence.In the case of RSM infection: loss of virulence.
RSM type phages may serve as a tool to control bacterial wilt.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (47 results)

All 2014 2013 2012 2011 Other

All Journal Article (10 results) (of which Peer Reviewed: 9 results,  Open Access: 2 results) Presentation (31 results) Remarks (4 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Insights into the diversity of φRSM phages infecting strains of the phytopathogen Ralstonia solanacearum complex : regulation and evolution2014

    • Author(s)
      Ahmed Askora, Takeru Kawasaki, Makoto Fujie and Takashi Yamada
    • Journal Title

      Mol. Genet. Genom.

      Volume: 289(in press) Issue: 4 Pages: 589-598

    • DOI

      10.1007/s00438-014-0835-3

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of Bacteriophages Cp1 and Cp2, the Strain-Typing Agents for Xanthomonas axonopodis pv. citri2014

    • Author(s)
      Abdelmonim Ali Ahmad, Megumi Ogawa, Takeru Kawasaki, Makoto Fujie and Takashi Yamada
    • Journal Title

      Appl. Environ. Microbiol.

      Volume: 80 Issue: 1 Pages: 77-85

    • DOI

      10.1128/aem.02310-13

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hyaluronan synthesis in cultured tobacco cells (BY-2) expressing a chlorovirus enzyme2013

    • Author(s)
      Rakkhumkaew, N., S. Shibatani, T. Kawasaki, M. Fujie, and T. Yamada
    • Journal Title

      Cytological studies. Biotech. Bioeng

      Volume: 110(4) Issue: 4 Pages: 1174-1179

    • DOI

      10.1002/bit.24783

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cryo-electron microscopy 3D structure of the jumbo phage RSL1 infecting the phytopathogen2013

    • Author(s)
      Effantin, G., R. Hamasaki, T. Kawasaki, M. Bacia, T. Yamada and G. Schoehn
    • Journal Title

      Ralstonia solanacearum Structure

      Volume: 21(2) Issue: 2 Pages: 298-305

    • DOI

      10.1016/j.str.2012.12.017

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prolonged synthesis of hyaluronan by Chlorella cells infected with chloroviruses2012

    • Author(s)
      Rakkhumkaew, N., T. Kawasaki, M. Fujie, and T. Yamada
    • Journal Title

      J. Biosci. Bioeng

      Volume: 115(5) Issue: 5 Pages: 527-531

    • DOI

      10.1016/j.jbiosc.2012.11.012

    • NAID

      110009611457

    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Journal Article] Utilization of Filamentous Phage φRSM3 to Control Bacterial Wilt Caused by Ralstonia solanacearum2012

    • Author(s)
      Hardian S. Addy, Ahmed Askora, Takeru Kawasaki, Makoto Fujie, and Takashi Yamada
    • Journal Title

      Plant Disease

      Volume: 96 Issue: 8 Pages: 1204-1209

    • DOI

      10.1094/pdis-12-11-1023-re

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Loss of Virulence of the Phytopathogen Ralstonia solanacearum Through Infection byφRSM Filamentous Phages2012

    • Author(s)
      Hardian S. Addy, Ahmed Askora, Takeru Kawasaki, Makoto Fujie, and Takashi Yamada
    • Journal Title

      PHYTOPATHOLOGY

      Volume: Vol. 102 Issue: 5 Pages: 469-477

    • DOI

      10.1094/phyto-11-11-0319-r

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The Filamentous PhageφRSS1 Enhances Virulence of Phytopathogenic Ralstonia solanacearum on Tomato2012

    • Author(s)
      Hardian S. Addy, Ahmed Askora, Takeru Kawasaki, Makoto Fujie, and Takashi Yamada
    • Journal Title

      PHYTOPATHOLOGY

      Volume: Vol. 102 Issue: 3 Pages: 241-245

    • DOI

      10.1094/phyto-10-11-0277

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Biocontrol of Ralstonia solanacearum by treatment with lytic bacteriophages2011

    • Author(s)
      Fujiwara A, Fujisawa M, Hamasaki R, Kawasaki T, Fujie M, Yamada T.
    • Journal Title

      Appl Environ Microbiol

      Volume: 77 Issue: 12 Pages: 4155-62

    • DOI

      10.1128/aem.02847-10

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Resolvase-like serine recombinase mediates integration/excision in the bacteriophageφRSM2011

    • Author(s)
      Ahmed Askora, Takeru Kawasaki, Makoto Fujie, and Takashi Yamada
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: VOL. 111 Issue: 2 Pages: 109-116

    • DOI

      10.1016/j.jbiosc.2010.10.001

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] シロイヌナズナを用いた植物ミオシン遺伝子の解析2013

    • Author(s)
      古川雄斗、辻翔平、川崎健、藤江誠、山田隆
    • Organizer
      Tomato Breeder's Roundtable 2013 Chiang Mai
    • Place of Presentation
      Thailand
    • Related Report
      2013 Final Research Report
  • [Presentation] 青枯病菌に感染するT7型ファージゲノムのダイナミックな再編成2013

    • Author(s)
      小寺星、藤原亜希子、川崎 健、藤江誠、山田隆
    • Organizer
      第65回日本生物工学会大会
    • Related Report
      2013 Annual Research Report
  • [Presentation] 青枯病菌に感染するT7型ファージの系統解析2013

    • Author(s)
      松浪美奈穂、川崎 健、藤江誠、山田隆
    • Organizer
      第65回日本生物工学会大会
    • Related Report
      2013 Annual Research Report
  • [Presentation] 青枯病菌ジャンボファージRSL1の構造と機能に関する研究2013

    • Author(s)
      竹内秀樹、川崎 健、藤江誠、山田隆
    • Organizer
      第65回日本生物工学会大会
    • Related Report
      2013 Annual Research Report
  • [Presentation] 青枯病菌繊維状ファージRSMの宿主制御機構に関する研究2013

    • Author(s)
      薮谷祐司
    • Organizer
      第65回日本生物工学会大会
    • Related Report
      2013 Annual Research Report
  • [Presentation] カンキツかいよう病菌に感染するファージの分離と解析:広島産レモンの保護2013

    • Author(s)
      小川恵、川崎 健、藤江誠、山田隆
    • Organizer
      第65回日本生物工学会大会
    • Related Report
      2013 Annual Research Report
  • [Presentation] クロレラがコードする2つの鞭毛関連遺伝子はストレス条件下で発現する

    • Author(s)
      熊谷徳泰、川崎健、藤江誠、山田隆
    • Related Report
      2013 Final Research Report
  • [Presentation] 青枯病菌に感染するT7型ファージゲノムのダイナミックな再編成

    • Author(s)
      小寺星, 藤原亜希子、川崎健、藤江誠、山田隆
    • Related Report
      2013 Final Research Report
  • [Presentation] 青枯病菌に感染するT7型ファージの系統解析

    • Author(s)
      松浪美奈穂、川崎健、藤江誠、山田隆
    • Related Report
      2013 Final Research Report
  • [Presentation] 青枯病菌ジャンボファージRSL1の構造と機能に関する研究

    • Author(s)
      竹内秀樹、川崎健、藤江誠、山田隆
    • Related Report
      2013 Final Research Report
  • [Presentation] 青枯病菌繊維状ファージRSMの宿主制御機構に関する研究

    • Author(s)
      藪谷祐司、川崎健、藤江誠、山田隆
    • Related Report
      2013 Final Research Report
  • [Presentation] カンキツかいよう病菌に感染するファージの分離と解析

    • Author(s)
      小川恵、川崎健、藤江誠、山田隆
    • Organizer
      広島産レモンの保護
    • Related Report
      2013 Final Research Report
  • [Presentation] Detection of the phytopathogen Ralstonia solanacearum using bacteriophages

    • Author(s)
      Takeru Kawasaki, Takashi Yamada
    • Related Report
      2013 Final Research Report
  • [Presentation] 青枯病菌に感染するT7型ファージゲノムのダイナミックな再編成

    • Author(s)
      小寺星、藤原亜希子、川崎健、藤江誠、山田隆
    • Related Report
      2013 Final Research Report
  • [Presentation] 青枯病菌に感染する3種のT7型ファージの系統解析と感染実験

    • Author(s)
      松浪美奈穂、川崎健、藤江誠、山田隆
    • Related Report
      2013 Final Research Report
  • [Presentation] バクテリオファージ耐性青枯病菌における変異遺伝子解析

    • Author(s)
      藤澤真理子、藤原亜希子、川崎健、藤江誠、山田隆
    • Related Report
      2013 Final Research Report
  • [Presentation] RSL型ジャンボファージの構造解析と持続的宿主抑制機構

    • Author(s)
      濱崎良介、藤原亜希子、川崎健、藤江誠、山田隆
    • Related Report
      2013 Final Research Report
  • [Presentation] RSS型繊維状ファージはXerCD/dif機構によって宿主ゲノムに組み込まれる

    • Author(s)
      田坂友一、川崎健、藤江誠、山田隆
    • Related Report
      2013 Final Research Report
  • [Presentation] Polonged Synthesis of Hyaluronan by Chlorella Cells Infected with Chloroviruses

    • Author(s)
      Numfon Rakkhumkaew, Shigeo Shibatani, Takeru Kawasaki, Makoto Fujie, Takashi Yamada
    • Related Report
      2013 Final Research Report
  • [Presentation] クロレラ鞭毛関連遺伝子の発現解析

    • Author(s)
      熊谷徳泰、川崎健、藤江誠、山田隆
    • Related Report
      2013 Final Research Report
  • [Presentation] クロレラウイルス遺伝子組換え技術の開発

    • Author(s)
      竹本有希、川崎健、藤江誠、山田隆
    • Related Report
      2013 Final Research Report
  • [Presentation] 青枯病菌Ralstonia solanacearumにおけるファージ耐性機構についての網羅的解析

    • Author(s)
      藤澤真理子、藤原亜希子、川崎健、藤江誠、山田隆
    • Related Report
      2013 Final Research Report
  • [Presentation] 青枯病菌の病原性を賦活化するファージRSSの遺伝子解析

    • Author(s)
      田坂友一、川崎健、藤江誠、山田隆
    • Related Report
      2013 Final Research Report
  • [Presentation] タバコ培養細胞BY-2を利用した青枯病菌感染モデル系の開発

    • Author(s)
      磯崎里奈、梶田浩未、川崎健、藤江誠、山田隆
    • Related Report
      2013 Final Research Report
  • [Presentation] 巨大ファージRSL1と青枯病菌Ralstonia solanacearumの相互作用に関する研究

    • Author(s)
      濱崎良介、藤原亜希子、川崎健、藤江誠、山田隆
    • Related Report
      2013 Final Research Report
  • [Presentation] Molecular mechanism of hyaluronan synthesis on tobacco cultured-cells (BY-2) exerted by chlorovirus enzymes

    • Author(s)
      Numfon Rakkhumkaew, Shigeo Shibatani, Takeru Kawasaki, Makoto Fujie, Takashi Yamada
    • Related Report
      2013 Final Research Report
  • [Presentation] 青枯病菌に感染するT7型ファージゲノムのダイナミックな再編成

    • Author(s)
      小寺 星、藤原 亜希子、川崎 健、藤江 誠、山田 隆
    • Organizer
      第64会日本生物工学会大会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] 青枯病菌に感染する3種のT7型ファージの系統解析と感染実験

    • Author(s)
      松波 美奈穂、川崎 健、藤江 誠、山田 隆
    • Organizer
      第64会日本生物工学会大会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] バクテリオファージ耐性青枯病菌における変異遺伝子解析

    • Author(s)
      藤澤 真理子、藤原 亜希子、川崎 健、藤江 誠、山田 隆
    • Organizer
      第64会日本生物工学会大会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] RSL型ジャンボファージの構造解析と持続的宿主抑制機構

    • Author(s)
      濱崎 良介、藤原 亜希子、川崎 健、藤江 誠、山田 隆
    • Organizer
      第64会日本生物工学会大会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] RSS型繊維状ファージはXerCD/dif機構によって宿主ゲノムに組み込まれる

    • Author(s)
      田坂 友一、川崎 健、藤江 誠、山田 隆
    • Organizer
      第64会日本生物工学会大会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://home.hiroshima-u.ac.jp/mbiotech/ichikou/

    • Related Report
      2013 Final Research Report
  • [Remarks] 広島大学大学院 先端研 生命分子情報学研究室(山田研) 一講ホームページへようこそ!

    • URL

      http://home.hiroshima-u.ac.jp/mbiotech/ichikou/index.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 広島大学大学院 先端研 生命分子情報学研究室(山田研) 一講ホームページへようこそ!

    • URL

      http://home.hiroshima-u.ac.jp/mbiotech/ichikou/

    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://home.hiroshima-u.ac.jp/mbiotech/ichikou/itikouindex.html

    • Related Report
      2011 Research-status Report
  • [Patent(Industrial Property Rights)] 青枯病予防剤および青枯病の予防方法2011

    • Inventor(s)
      山田 隆、藤江 誠、川崎 健
    • Industrial Property Rights Holder
      広島大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2011-04-28
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 青枯病予防剤および青枯病の予防方法の開発2011

    • Inventor(s)
      藤江 誠、山田 隆、川崎 健
    • Industrial Property Rights Holder
      藤江 誠、山田 隆、川崎 健
    • Industrial Property Number
      2011-102153
    • Filing Date
      2011
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-12-27  

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