Identification and functional analysis of O-glycosylated proteins in filamentous fungi

• Project/Area Number: 23780084
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Kyushu University
• Principal Investigator: FUTAGAMI Taiki 九州大学, 大学院・農学研究院, 助教 (60512027)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: O-結合型糖鎖 / 細胞壁ストレス / 糸状菌 / Aspergillus / Stress sensor / Cell wall integrity / Congo red / MidB / AgsB / 細胞壁合成 / Mid2 / kre9

Research Abstract

O-glycosylated proteins play an important role in the cell morphogenesis of fungal species. We characterized the putative stress sensor protein ofcell wall integrity (CWI) signaling, Mid2-like protein (MidB), in the Aspergillus nidulans. The disruption of midBshowed the increased sensitivities to the cell wall inhibitors such as Congo red and calcofluor, indicating that the MidB is involved in the CWI in the A. nidulans. However, the MidB was not essential for the downstream MpkA-RlmA signaling that caused a transient transcriptional up-regulation of the agsBgene.

Research Products

• [Journal Article] Putative cell wall integrity sensor proteins in Aspergillus nidulans (2012)
  • Author(s): Futagami T, Goto M
  • Journal Title: Commun Integr Biol Volume: 5 Issue: 2 Pages: 206-208
  • DOI: 10.4161/cib.18993
  • Peer Reviewed
• [Journal Article] Putative cell wall integrity sensor proteins in Aspergillus nidulans (2012)
  • Author(s): Taiki Futagami, Masatoshi Goto
  • Journal Title: Communicative and Integrative Biology Volume: 5
  • Peer Reviewed
• [Presentation] Aspergillus nidulansにおける糖転移酵素 PmtC 基質タンパク質の同定 (2012)
  • Author(s): 上原拓磨、二神泰基、豊浦利枝子、岩下和裕、梶原康博、高下秀春、大森俊郎、竹川薫、後藤正利
  • Organizer: 第19回日本生物工学会九州支部大会
  • Place of Presentation: 別府大学
  • Year and Date: 2012-12-01
• [Presentation] 糸状菌に特異的なストレス応答機構に関する研究 (2012)
  • Author(s): 二神泰基
  • Organizer: 第15回真核微生物交流会
  • Place of Presentation: 酒類総合研究所
  • Year and Date: 2012-06-29
• [Presentation] Aspergillus nidulansにおける Mid2 様タンパク質の機能解析 (2012)
  • Author(s): 二神泰基、瀬戸和史、梶原康博、高下秀春、大森俊郎、竹川薫、後藤正利
  • Organizer: 第64回日本生物工学会大会
  • Place of Presentation: 神戸国際会議場
• [Presentation] Aspergillus nidulansにおけるMid2様タンパク質の機能解析 (2012)
  • Author(s): 二神泰基、瀬戸和史、梶原康博、高下秀春、大森俊郎、竹川薫、後藤正利
  • Organizer: 第64回日本生物工学会大会
  • Place of Presentation: 神戸国際会議場
• [Presentation] Aspergillus nidulansにおける糖転移酵素PmtC基質タンパク質の同定 (2012)
  • Author(s): 上原拓磨、二神泰基、豊浦利枝子、岩下和裕、梶原康博、高下秀春、大森俊郎、竹川薫、後藤正利
  • Organizer: 第19回日本生物工学会九州支部大会
  • Place of Presentation: 別府大学
• [Presentation] Aspergillus nidulansにおけるkre9ホモログ遺伝子の機能解析 (2011)
  • Author(s): 上原拓磨、瀬戸和史、二神泰基、大森俊郎、竹川薫、後藤正利
  • Organizer: 日本農芸化学会西日本支部・中四国支部合同大会
  • Place of Presentation: 宮崎大学
• [Presentation] Aspergillus nidulans におけるkre9 ホモログ遺伝子の機能解析. (2011)
  • Author(s): 上原拓磨、瀬戸和史、二神泰基、大森俊郎、竹川薫、後藤正利
  • Organizer: 日本農芸化学会西日本支部・中四国支部合同大会
  • Place of Presentation: 宮崎大学