Development of one-step protein expression and purification systemUsing silica particles
Project/Area Number |
23780085
|
Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Applied microbiology
|
Research Institution | Kyushu University |
Principal Investigator |
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | シリカ / タンパク質発現 / Thermus / promoter / silica / over-expression |
Research Abstract |
The aim of this study is to develop novel protein expression and purification system using silica-inducible promoter and silica-binding domain. Transcriptional analysis revealed that iron deficient caused by colloidal silica activated this promoter and ferric uptake regulator (Fur: TTHA0255) regulated this promoter. Almost the same phenomenon was seen in E. coli, and we have succeeded in the construction of silica-inducible expression vector. However, conditions for one-step expression and purification have not completed.
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Report
(3 results)
Research Products
(4 results)