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Microbial ecology of nitrous oxide-reducing denitrifying bacteria in the livestock waste composts

Research Project

Project/Area Number 23780087
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied microbiology
Research InstitutionTohoku Gakuin University

Principal Investigator

OHTSUBO Wakako  東北学院大学, 工学総合研究所, 研究員 (00598203)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords亜酸化窒素 / 堆肥化プロセス / 脱窒 / 群集構造解析 / アンモニア酸化 / 細菌群集構造解析
Research Abstract

In this study we performed composting experiments using solidwastes from piggery wastewater treatment processes. Our results indicated that the timing of N_2O emission follows emergence of ammonia-oxidizing bacteria, which produce denitrification substrates such as nitrate and nitrite. We also found that hyperthermophilic pre-treatment reduces N_2O emission from composting, which is suggest to have been caused by denitrifying bacteria with N_2O-reducing activities. Phylogenetic analyses showed that community structures of ammonia-oxidizing bacteria and denitrifying bacteria dynamically changed as composting proceeded. Denitrifying bacterial strains isolated from our compost samples were able to perform denitrification under oxic or microoxic conditions. These findings collectively suggest that N_2O emission patterns during composting is strongly affected by community structures and functions of ammonia-oxidizing bacteria and denitrifying bacteria, which should be effectively controlled to reduce N_2O emission from composting processes.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (21 results)

All 2013 2012 2011

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (18 results)

  • [Journal Article] Community structure and population dynamics of ammonia oxidizers in composting processes of ammonia-rich livestock waste.2013

    • Author(s)
      Yamada, T., Araki, S., Ikeda-Ohtsubo, W., Okamura, K., Hiraishi, A., Ueda, H., Ueda, Y., Miyauchi, K., and Endo, G.
    • Journal Title

      Systematic and Applied Microbiology

      Volume: in press Issue: 5 Pages: 359-67

    • DOI

      10.1016/j.syapm.2013.02.001

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] コンポスト製造過程において発生する亜酸化窒素の削減に関与する脱窒細菌遺伝子の多様性と消長2011

    • Author(s)
      進藤絵里香、大坪和香子、上田英代、上田裕一、宮内啓介、遠藤銀朗
    • Journal Title

      土木学会論文集 G(環境)

      Volume: 67(7) Pages: 441-448

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] コンポスト製造過程において発生する亜酸化窒素の削減に関与する脱窒細菌遺伝子の多様性と消長2011

    • Author(s)
      進藤絵里香、大坪和香子、上田裕一、上田英代、宮内啓介、遠藤銀朗
    • Journal Title

      土木学会論文集G(環境)

      Volume: Vol. 67, No. 7 Pages: 441-448

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] コンポスト製造過程で出現する脱窒細菌が保有する N2O 還元酵素の多様性に関する研究2013

    • Author(s)
      東海林晃、大坪和香子、宮内啓介、遠藤銀朗
    • Organizer
      平成24年度土木学会東北支部 技術研究発表会
    • Place of Presentation
      仙台
    • Related Report
      2012 Final Research Report
  • [Presentation] 堆肥化プロセスにおける亜酸化窒素(N_2O) 発生に硝化・脱窒細菌が与える影響2013

    • Author(s)
      大坪和香子、進藤絵里香、宮内啓介、遠藤銀朗
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      仙台
    • Related Report
      2012 Final Research Report
  • [Presentation] コンポスト製造過程で出現する脱窒細菌が保有するN2O 還元酵素の多様性に関する研究2013

    • Author(s)
      東海林晃
    • Organizer
      平成24年度土木学会東北支部 技術研究発表会
    • Place of Presentation
      東北大学川内キャンパス、仙台
    • Related Report
      2012 Annual Research Report
  • [Presentation] 堆肥化プロセスにおける亜酸化窒素(N2O) 発生に硝化・脱窒細菌が与える影響2013

    • Author(s)
      大坪和香子
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学川内キャンパス、仙台
    • Related Report
      2012 Annual Research Report
  • [Presentation] 好気的条件下で脱窒を行う能力を有する堆肥由来細菌の単離と同定2012

    • Author(s)
      大坪和香子、尾形裕明、宮内啓介、遠藤銀朗
    • Organizer
      日本農芸化学会 2012年度大会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] Nitrous oxide (N_2O) emission during composting: when and how?2012

    • Author(s)
      大坪和香子
    • Organizer
      Japan-Finland Biotechnology Symposium 2012
    • Place of Presentation
      仙台
    • Related Report
      2012 Final Research Report
  • [Presentation] Effect of a hyperthermophilic pretreatment on nitrous oxide emission-pattern and populationdynamics of nitrifying and denitrifying bacteria during composting of swine waste2012

    • Author(s)
      Wakako Ikeda-Ohtsubo, Erika Shindo, Keisuke Miyauchi, Ginro Endo
    • Organizer
      14th International Symposium on Microbial Ecology (ISME-14)
    • Place of Presentation
      コペンハーゲン、デンマーク
    • Related Report
      2012 Final Research Report
  • [Presentation] Effect of mismatches in primers for amplifying nitrous oxide reductase (nosZ) genes on communitystructure analysis of denitrifying bacteria2012

    • Author(s)
      Wakako Ikeda-Ohtsubo, Erika Shindo, Keisuke Miyauchi, Ginro Endo
    • Organizer
      第28回日本微生物生態学会大会
    • Place of Presentation
      豊橋
    • Related Report
      2012 Final Research Report
  • [Presentation] Nitrous oxide (N2O) emission during composting – when and how?2012

    • Author(s)
      Wakako Ikeda-Ohtsubo
    • Organizer
      Japan-Finland Biotechnology Symposium 2012
    • Place of Presentation
      東北学院大学土樋キャンパス、仙台
    • Related Report
      2012 Annual Research Report
  • [Presentation] Effect of a hyperthermophilic pretreatment on nitrous oxide emission-pattern and population dynamics of nitrifying and denitrifying bacteria during composting of swine waste2012

    • Author(s)
      Wakako Ikeda-Ohtsubo
    • Organizer
      14th International Symposium on Microbial Ecology (ISME-14)
    • Place of Presentation
      コペンハーゲン、デンマーク
    • Related Report
      2012 Annual Research Report
  • [Presentation] Effect of mismatches in primers for amplifying nitrous oxide reductase (nosZ) genes on community structure analysis of denitrifying bacteria2012

    • Author(s)
      大坪和香子
    • Organizer
      第28回日本微生物生態学会大会
    • Place of Presentation
      豊橋技術科学大学、豊橋
    • Related Report
      2012 Annual Research Report
  • [Presentation] 好気的条件下で脱窒を行う能力を有する堆肥由来細菌の単離と同定2012

    • Author(s)
      大坪和香子、尾形裕明、宮内啓介、遠藤銀朗
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都市(京都府)
    • Related Report
      2011 Research-status Report
  • [Presentation] Quantification and diversity analysis of denitrifying enzyme genes in livestock waste compost2011

    • Author(s)
      Wakako Ikeda-Ohtsubo, Erika Shindo, Hideyo Ueda, Yasuichi Ueda, Keisuke Miyauchi, Ginro Endo
    • Organizer
      Enzymes in the Environment
    • Place of Presentation
      Bad Nauheim, ドイツ
    • Related Report
      2012 Final Research Report
  • [Presentation] Isolation of aerobic denitrifying bacteria from livestock waste compost2011

    • Author(s)
      Wakako Ikeda-Ohtsubo, Erika Shindo, Keisuke Miyauchi, Ginro Endo
    • Organizer
      XIII International Congress of Bacteriology and AppliedMicrobiology (IUMS 2011)
    • Place of Presentation
      札幌
    • Related Report
      2012 Final Research Report
  • [Presentation] 養豚場排水処理残渣の堆肥化プロセスに存在する脱窒細菌群集の定量および系統解析2011

    • Author(s)
      大坪和香子、進藤絵里香、上田裕一、上田英代、宮内啓介、遠藤銀朗
    • Organizer
      第27回日本微生物生態学会大会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] Quantification and diversity analysis of denitrifying enzyme genes in livestock waste compost2011

    • Author(s)
      Wakako Ikeda-Ohtsubo, Erika Shindo, Hideyo Ueda, Yasuichi Ueda, Keisuke Miyauchi, Ginro Endo
    • Organizer
      Enzymes in the Environment: Activity, Ecology & Applications
    • Place of Presentation
      Bad Nauheim, Germany
    • Related Report
      2011 Research-status Report
  • [Presentation] Isolation of aerobic denitrifying bacteria from livestock waste compost2011

    • Author(s)
      Wakako Ikeda-Ohtsubo, Erika Shindo, Keisuke Miyauchi, Ginro Endo
    • Organizer
      XIII International Congress of Bacteriology and Applied Microbiology (IUMS 2011)
    • Place of Presentation
      札幌市(北海道)
    • Related Report
      2011 Research-status Report
  • [Presentation] 養豚場排水処理残渣の堆肥化プロセスに存在する脱窒細菌群集の定量および系統解析2011

    • Author(s)
      進藤絵里香、大坪和香子、上田裕一、上田英代、宮内啓介、遠藤銀朗
    • Organizer
      第27回日本微生物生態学会大会
    • Place of Presentation
      京都市(京都府)
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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