Heterologous secondary metabolite production in genome-minimized Streptomyces host
| Project/Area Number |
23780089
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Kitasato University |
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Principal Investigator |
KOMATSU Mamoru 北里大学, 大学院・感染制御科学府, 助教 (40414057)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | Sterptomyces avermitilis / Cre/loxP / genome / secondary metabolism / actinophage / synthetic biology / Streptomyces avermitilis |
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| Research Abstract |
We have already demonstrated the heterologous expression of biosynthetic gene clusters in the large-deletion mutants of S. avermitilis (SUKA) that lack major endogenous biosynthetic gene clusters and did not produce these endogenousmetabolites. SUKA have been shown to be versatile and effective hosts for the expression of heterologous gene clusters governing the production of a variety of secondary metabolites. In this study we developed novel marker-less multi gene introduction system using 5 integrating vectors derived from 5 different actinophages. It is useful gene introduction system for production of unnatural compounds by combinatorial biosynthesis.
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Report
(3 results)
Research Products
(38 results)
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[Journal Article] Engineered Streptomyces Avermitilis Host for Heterologous Expression of Biosynthetic Gene Cluster for Secondary Metabolites2013
Author(s)
M. Komatsu, K. Komatsu, H. Koiwai, Y. Yarnada, I. Kozone, M. Izumikawa, J. Hashimoto, M. Takagi, S. Omura, K. Shin-ya, D. E. Cane, and H. Ikeda
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Journal Title
ACS Synth. Biol.
Volume: 2
Issue: 7
Pages: 384-396
DOI
Related Report
Peer Reviewed
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[Journal Article] Site-specific genome integration in alphaproteobacteria mediated by TG1 integrase2012
Author(s)
Morita, K., Morimura, K., Fusada, N., Komatsu, M., Ikeda, H., Hirano, N., Takahashi, H
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Journal Title
Appl. Microbiol. Biotechnol
Volume: 93
Issue: 1
Pages: 295-304
DOI
Related Report
Peer Reviewed
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[Journal Article] The autoregulator-receptor homologue AvaR3 plays a regulatory role in antibiotic production, mycelial aggregation and colony development of Streptomyces avermitilis2011
Author(s)
Miyamoto, K., Kitani, S., Komatsu, M., Ikeda, H., Nihira, T.
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Journal Title
Microbiology
Volume: 157
Issue: 8
Pages: 2266-2275
DOI
Related Report
Peer Reviewed
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