The studies for the regulatory mechanism of lipA and for function of the surfactant protein, EliA, in Ralstonia sp. NT80.
Project/Area Number |
23780090
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Applied microbiology
|
Research Institution | Chuo University |
Principal Investigator |
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | Lipase / EliA / PHB / Ralstonia / Stearyl alcohol / リパーゼ / 高級アルコール / 分泌蛋白質 / プロテオーム / 遺伝子発現制御 |
Research Abstract |
Stearyl alcohol induced not only the expression of lipase and infection-related secreted proteins but also the synthesis of PHB in Ralstonia sp. NT80. The secreted protein, EliA, was also induced by stearyl alcohol and facilitated induction of lipase expression. The content of stearyl alcohol in the culture supernatant was reduced by wild-type cells but not by eliA cells. It is most likely that EliA facilitates the induction of lipase expression, by promoting the recognition and/or incorporation of stearyl alcohol.
|
Report
(4 results)
Research Products
(29 results)