| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Research Abstract |
Saccharomyces cerevisiae strain YFY21 engineered of both of N- and O-glycan, exhibits a sever growth defect. YFY22, which was isolated from YFY21, had normal growth. The gene expression profiles of YFY21 and YFY22 were analyzed using DNA microarrays. Expression of genes that encodes enzymes in the glycolysis was up-regulated in YFY22.
Additionally mutated pmt2pmt4 disruptant strain exhibits the growth defect lacking phenotype of pmt2pmt4 disruptant . In the mutated pmt2pmt4 disruptant , Wsc1 was not processed and O-mannosylation of mannoprotein on cell wall increased. These results showed that up-regulation of glucose metabolism and protection against protein-degradation by addition of O-mannosylation are essential role for restored growth.
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