Elucidation of production mechanism of peptidyl-tRNAs dropped from ribosomes in bacterial translation system

Research Project

Project/Area Number	23780102
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Applied biochemistry
Research Institution	The University of Tokyo
Principal Investigator	NAGAO Asuteka 東京大学, 大学院・工学系研究科, 助教 (30588017)
Project Period (FY)	2011 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords	遺伝子発現 / 翻訳 / 翻訳精度維持機構 / tRNA / リボソーム / rRNA
Research Abstract	We directly analyzed and characterized peptidyl-tRNAs which were dissociated from the ribosome during elongation by using LC/MS analyses and RNA isolation technique. When each individual pep-tRNA was isolated and analyzed in detail, we found that noncognate peptidyl-tRNAs whose nascent chains didn’t match any of E.coli genes were also dropped from the ribosome. This suggested a novel quality control mechanism after peptidyltransfer reaction, namely noncognate peptidyl-tRNAs can berejected from the ribosome.

Report

(3 results)