Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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Research Abstract |
We directly analyzed and characterized peptidyl-tRNAs which were dissociated from the ribosome during elongation by using LC/MS analyses and RNA isolation technique. When each individual pep-tRNA was isolated and analyzed in detail, we found that noncognate peptidyl-tRNAs whose nascent chains didn’t match any of E.coli genes were also dropped from the ribosome. This suggested a novel quality control mechanism after peptidyltransfer reaction, namely noncognate peptidyl-tRNAs can berejected from the ribosome.
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