Elucidation of production mechanism of peptidyl-tRNAs dropped from ribosomes in bacterial translation system
| Project/Area Number |
23780102
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAGAO Asuteka 東京大学, 大学院・工学系研究科, 助教 (30588017)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 遺伝子発現 / 翻訳 / 翻訳精度維持機構 / tRNA / リボソーム / rRNA |
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| Research Abstract |
We directly analyzed and characterized peptidyl-tRNAs which were dissociated from the ribosome during elongation by using LC/MS analyses and RNA isolation technique. When each individual pep-tRNA was isolated and analyzed in detail, we found that noncognate peptidyl-tRNAs whose nascent chains didn’t match any of E.coli genes were also dropped from the ribosome. This suggested a novel quality control mechanism after peptidyltransfer reaction, namely noncognate peptidyl-tRNAs can berejected from the ribosome.
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Report
(3 results)
Research Products
(5 results)
