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Elucidation of molecular functions of the oxygen-evolving complex homologs in chloroplasts

Research Project

Project/Area Number 23780103
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied biochemistry
Research InstitutionKyoto University

Principal Investigator

IFUKU KENTARO  京都大学, 生命科学研究科, 助教 (50359783)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords植物 / 光合成 / 電子伝達 / 環境応答 / 分子進化 / 葉緑体 / 電子伝達鎖 / 光化学系II / 酸素発生系 / 循環的電子伝達
Research Abstract

The oxygen-evolving complex (OEC) proteins are membrane-peripheral proteins bound to thylakoid lumenal side of photosystem (PS) II, and PsbP and PsbQ are the OEC proteins found in green plants. In addition, multiple homologs for PsbP and PsbQ have been identified in chloroplasts and in cyanobacteria; however, their functions have not been fully elucidated yet. In this study, we characterized molecular functions of the OEC homologs using mutants of Arabidopsis and cyanobacteria. It was found that the OEC homologs indeed have various functions in photosynthetic electron transport, such as the regulation of PSII activity under various light conditions and the accumulation of the chloroplast NAD(P)H dehydrogenase (NDH) complex that operates in cyclic electron transport around PSI. These results suggest that functional diversification of the OEC homologs has occurred during evolution to support the optimum photosynthesis.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (41 results)

All 2014 2013 2012 2011 Other

All Journal Article (11 results) (of which Peer Reviewed: 7 results) Presentation (29 results) (of which Invited: 8 results) Remarks (1 results)

  • [Journal Article] Cross-Linking Evidence for Multiple Interactions of the PsbP and PsbQ Proteins in a Higher Plant Photosystem II Supercomplex2014

    • Author(s)
      Ido, K., Nield, J., Fukao, Y., Nishimura, T., Sato, F., Ifuku, K
    • Journal Title

      J. Biol. Chem

      Volume: (in press)

    • Related Report
      2013 Final Research Report
  • [Journal Article] Function and association of CyanoP in photosystem II of Synechocystis sp. PCC 68032014

    • Author(s)
      Aoi, M., Kashino, Y., Ifuku, K.
    • Journal Title

      Res. Chem. Intermed

      Volume: (in press)

    • Related Report
      2013 Final Research Report
  • [Journal Article] The PsbP and PsbQ family proteins in the photosynthetic machinery of chloroplasts2014

    • Author(s)
      Kentaro Ifuku
    • Journal Title

      Plant Physiol. Biochem.

      Volume: XX Pages: 108-114

    • DOI

      10.1016/j.plaphy.2014.01.001

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the basic amino acid residues on the PsbP protein involved in the electrostatic interaction with photosystem II2014

    • Author(s)
      T. Nishimura, C. Uno, K. Ido, R. Nagao, T. Noguchi, F. Sato and K. Ifuku
    • Journal Title

      Biochim. Biophys. Acta

      Volume: (in press) Issue: 9 Pages: 1447-1453

    • DOI

      10.1016/j.bbabio.2013.12.012

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Function and association of CyanoP in photosystem II of Synechocystis sp. PCC 68032014

    • Author(s)
      Masaki Aoi, Yasuhiro Kashino, Kenatro Ifuku
    • Journal Title

      Research on Chemical Intermediates

      Volume: XX

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 光化学系IIの光阻害に対するチラコイド膜内腔タンパク質の役割2013

    • Author(s)
      伊福健太郎
    • Journal Title

      光合成研究23

      Volume: (2) Pages: 86-93

    • URL

      http://photosyn.jp/journal.html

    • Related Report
      2013 Final Research Report
  • [Journal Article] Functions of Thylakoid Lumenal Proteins Against Photoinhibiton of Photosystem II2013

    • Author(s)
      Kentaro Ifuku
    • Journal Title

      光合成研究

      Volume: 23(2) Pages: 86-93

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure around conserved His144 and Asp165 in the PsbP protein is important for the interaction between its N-terminus and the Cyt b559 subunit of photosystem II2012

    • Author(s)
      K. Ido, S. Kakiuchi, C. Uno, T. Nishimura, Y. Fukao, T. Noguchi, F. Sato, and K. Ifuku
    • Journal Title

      J. Biol. Chem

      Volume: 287 Issue: 31 Pages: 26377-26387

    • DOI

      10.1074/jbc.m112.385286

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The PsbQ protein stabilizes the functional binding of the PsbP protein to photosystem II in higher plants2012

    • Author(s)
      S. Kakiuchi, C. Uno, K. Ido, T. Nishimura, T. Noguchi, K. Ifuku, and F. Sato
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1817 Issue: 8 Pages: 1346-1351

    • DOI

      10.1016/j.bbabio.2012.01.009

    • Related Report
      2013 Final Research Report 2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 葉緑体酸素発生系タンパク質の分子進化と植物の環境適応2012

    • Author(s)
      伊福健太郎
    • Journal Title

      生化学

      Volume: 84(11) Pages: 934-938

    • NAID

      10031136516

    • URL

      http://www.jbsoc.or.jp/mini_review

    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Journal Article] Structure of the chloroplast NADH dehydrogenase-like complex : nomenclature for nuclear-encoded subunits2011

    • Author(s)
      Ifuku K, Endo T, Shikanai T, Aro E-M
    • Journal Title

      Plant Cell Physiol

      Volume: 52 Issue: 9 Pages: 1560-1568

    • DOI

      10.1093/pcp/pcr098

    • NAID

      10029714253

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] PsbP-Like protein 1 (PPL1)の光化学系II超複合体における分子機能2014

    • Author(s)
      草間翔子,松井信太郎,佐藤文彦,伊福健太郎
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Final Research Report
  • [Presentation] 高等植物の光化学系II膜表在性サブユニットの結合様式2014

    • Author(s)
      西村太志,宇野千尋,井戸邦夫,長尾遼,深尾陽一郎,野口巧,佐藤文彦,伊福健太郎
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Final Research Report
  • [Presentation] Binding of the extrinsic subunits in the higher plant PSII complex2014

    • Author(s)
      Taishi Nishimura, Chihiro Uno, Kunio Ido, Ryo Nagao, Yoichiro Fukao, Takumi Noguchi, Fumihiko Sato, Kentaro Ifuku
    • Organizer
      第55回 日本植物生理学会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] PsbP-Like protein 1 (PPL1)の光化学系II超複合体における分子機能2014

    • Author(s)
      草間 翔子、松井 信太郎、佐藤 文彦、伊福 健太郎
    • Organizer
      第55回 日本植物生理学会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 葉緑体酸素発生系タンパク質の分子進化と機能分化2013

    • Author(s)
      伊福健太郎
    • Organizer
      第27回インターゲノミクスセミナー
    • Place of Presentation
      神戸大学
    • Year and Date
      2013-12-06
    • Related Report
      2013 Final Research Report
  • [Presentation] チラコイド膜タンパク質複合体の機能を支える膜表在性タンパク質の役割2013

    • Author(s)
      伊福健太郎
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      札幌
    • Year and Date
      2013-09-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Interaction and function of the membrane-extrinsic proteins of photosystem II in higher plants2013

    • Author(s)
      Ifuku, K
    • Organizer
      International Conference, Photosynthesis Research for Sustainability
    • Place of Presentation
      Baku, Azerbijan
    • Year and Date
      2013-06-06
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Functions of Thylakoid Lumenal Proteins Against Photoinhibiton of Photosystem II2013

    • Author(s)
      Ifuku, K.
    • Organizer
      第54回日本植物生理学会年会シンポジウム
    • Place of Presentation
      岡山
    • Year and Date
      2013-03-23
    • Related Report
      2013 Final Research Report
  • [Presentation] 緑色植物型の膜表在性タンパク質による光化学系II水分解反応の制御機構2013

    • Author(s)
      伊福健太郎
    • Organizer
      日本植物生理学会シンポジウム
    • Place of Presentation
      岡山
    • Year and Date
      2013-03-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Interaction of the PsbP protein in higher plant PSII complex2013

    • Author(s)
      Nishimura, T., Asada, M., Ido, K., Mino, H., Sato, F., and Ifuku, K
    • Organizer
      The 16^<th> International Congress on Photosynthesis
    • Place of Presentation
      St. Louis, MO, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional analysis of PsbP-like protein 1 (PPL1) in the PSII-LHCII supercomplex2013

    • Author(s)
      Kusama, S., Matsui, S., Sato, F., Ifuku, K
    • Organizer
      The 16^<th> International Congress on Photosynthesis
    • Place of Presentation
      St. Louis, MO, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] シアノバクテリア型PsbP(Cyano P)の分子機能に関する研究2013

    • Author(s)
      青井政樹、菓子野康浩、佐藤文彦、伊福健太郎
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Related Report
      2013 Final Research Report
  • [Presentation] PsbP-Like protein 1(PPL1)の発現抑制が光化学系II複合体形成に与える影響2013

    • Author(s)
      草間翔子、松井信太郎、佐藤文彦、伊福健太郎
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Related Report
      2013 Final Research Report
  • [Presentation] 光化学系II光阻害感受性植物における光合成馴化機構の解析2013

    • Author(s)
      山口咲希、井戸邦夫、遠藤剛、佐藤文彦、伊福健太郎
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Related Report
      2013 Final Research Report
  • [Presentation] 光化学系II光阻害感受性植物における光合成馴化機構の解析2013

    • Author(s)
      山口 咲希、井戸 邦夫、遠藤 剛、佐藤 文彦、伊福 健太郎
    • Organizer
      第54回 日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Research-status Report
  • [Presentation] シアノバクテリア型PsbP(Cyano P)の分子機能に関する研究2013

    • Author(s)
      青井政樹、菓子野康浩、佐藤文彦、伊福健太郎
    • Organizer
      第54回 日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Research-status Report
  • [Presentation] PsbP-Like protein 1 (PPL1)の発現抑制が光化学系II複合体形成に与える影響2013

    • Author(s)
      草間翔子、松井信太郎、佐藤文彦、伊福健太郎
    • Organizer
      第54回 日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 強光ストレス耐性に関わるPsbP-Like 1 (PPL1)の分子機能解析2012

    • Author(s)
      松井信太郎,石原靖子,井戸邦夫,佐藤文彦,伊福健太郎
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大
    • Year and Date
      2012-03-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Evolution of the OEC family proteins in chloroplasts and plant adaptation to the environment2012

    • Author(s)
      Ifuku, K.
    • Organizer
      Japan-Finnish Seminar 2012
    • Place of Presentation
      Naantali, Finland
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 光合成電子伝達鎖を制御する葉緑体酸素発生系タンパク質の分子機能に関する研究2012

    • Author(s)
      伊福健太郎*
    • Organizer
      日本農芸化学会関西支部会第473回講演会(招待講演)
    • Place of Presentation
      京都大学学友会館
    • Related Report
      2011 Research-status Report
  • [Presentation] 強光ストレス耐性に関わる PsbP-Like 1 (PPL1) の分子機能解2012

    • Author(s)
      松井信太郎, 石原靖子, 井戸邦夫, 佐藤文彦, 伊福健太郎*
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学
    • Related Report
      2011 Research-status Report
  • [Presentation] Functional Diversification of photosystem II extrinsic subunits in higher plants2011

    • Author(s)
      Ifuku, K
    • Organizer
      International Conference, Photosynthesis Research for Sustainability
    • Place of Presentation
      Baku, Azerbijan
    • Year and Date
      2011-07-27
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 光合成電子伝達鎖を制御する葉緑体酸素発生系タンパク質の分子機能に関する研究2011

    • Author(s)
      伊福健太郎*
    • Organizer
      2011年度日本農芸化学会関西・中部支部合同大会(招待講演)
    • Place of Presentation
      京都大学農学部
    • Related Report
      2011 Research-status Report
  • [Presentation] 葉緑体酸素発生系タンパク質の分子進化と機能分化

    • Author(s)
      伊福 健太郎
    • Organizer
      第27回インターゲノミクスセミナー
    • Place of Presentation
      神戸大学農学部
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] チラコイド膜タンパク質複合体機能を支える膜表在性タンパク質の役割

    • Author(s)
      伊福 健太郎
    • Organizer
      日本植物学会
    • Place of Presentation
      北海道大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] INTERACTION AND FUNCTION OF THE MEMBRANE-EXTRINSIC PROTEINS OF PHOTOSYSTEM II IN HIGHER PLANTS

    • Author(s)
      Kentaro Ifuku
    • Organizer
      International Conference, Photosynthesis Research for Sustainability
    • Place of Presentation
      Baku, Azerbaijian
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Evolution of the OEC family proteins in chloroplasts and plant adaptation to the environment

    • Author(s)
      Kentaro Ifuku
    • Organizer
      Japan-Finnish Seminar 2012
    • Place of Presentation
      Naantali, Finland
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Molecular function of PPL1 in the PSII-LHCII supercomplex

    • Author(s)
      Shoko Kusama, Shintaro Matsui, Fumihiko Sato, Kentaro Ifuku
    • Organizer
      Okayama Univ. International Symposium “Structure and Dynamics of Photosynthetic Systems”
    • Place of Presentation
      Okayama, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] Functions of Thylakoid Lumenal Proteins Against Photoinhibiton of Photosystem II

    • Author(s)
      Kentaro Ifuku, Shintaro Matsui, Shoko kusama, Fumihiko Sato
    • Organizer
      第54回日本植物生理学会年会 シンポジウム
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Research-status Report
    • Invited
  • [Remarks]

    • URL

      http://www.lif.kyoto-u.ac.jp/labs/callus/

    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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