Analysis of regulatory mechanisms of C/N-balance by plastid signaling using sugar-sensitive mutants
Project/Area Number |
23780108
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Applied biochemistry
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Research Institution | Kinki University |
Principal Investigator |
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Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 植物生化学 / C/Nバランス / 糖シグナル / インベルターゼ / 葉緑体 |
Research Abstract |
We recently identified an Arabidopsis gain-of-function mutant of plastidic invertase (INV-E), sicy-192, as a sucrose sensitive mutant, and found that INV-E participates in regulating the carbon/nitrogen (C/N) balance. Interestingly, PGE was suppressed in the mutants on treatment with sucrose, suggesting that INV-E regulates C/N balance via the plastid signaling. We created and analyzed double mutants of sicy-192 and genome uncoupled 1 (gun1-101), which lacks GUN1, a master regulator of the plastid signaling. On treatment with sucrose, the expression of photosynthesis- and nitrogen assimilation-related genes was lower and higher, respectively, in the sicy-192 mutants compared to the wild-type plants. Interestingly, the perturbation of C/N balance was recovered by the disruption of GUN1, suggesting an involvement of INV-E in the GUN1-mediated plastid signaling.
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Report
(3 results)
Research Products
(17 results)